Figure 4From: Effect of amorphous silica nanoparticles on in vitro RANKL-induced osteoclast differentiation in murine macrophages Number of osteoclasts induced by RANKL and silica particles in RAW264.7. RAW264.7 cells were incubated with 10 μg/ml silica particles and 30 ng/ml RANKL for 5 days. The number of TRAP-positive multinucleate cells (> 3 nuclei) were counted as osteoclasts in three different areas. Data are expressed as the mean ± SD (n = 3).Back to article page