Figure 7

GO-promoted cell death of splenic erythroid cells. FACS analysis of proportion of apoptotic erythroid cells (Ter119⁺ cell population). The single-cell suspensions from spleens were simultaneously stained with PE-conjugated anti-Ter119 Ab, FITC-conjugated Annexin V, and 7AAD to sort the apoptotic Ter119⁺ in spleens. After sorting in the first left gate, Ter119 positive cells were selected and then further analyzed for cell death. The quantified data for the average percentage of apoptotic Ter119⁺ cells are shown in the bar graph (n = 4).