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Synthesis of silver nanoparticles using reducing agents obtained from natural sources (Rumex hymenosepalus extracts)
Nanoscale Research Lettersvolume 8, Article number: 318 (2013)
We have synthesized silver nanoparticles from silver nitrate solutions using extracts of Rumex hymenosepalus, a plant widely found in a large region in North America, as reducing agent. This plant is known to be rich in antioxidant molecules which we use as reducing agents. Silver nanoparticles grow in a single-step method, at room temperature, and with no addition of external energy. The nanoparticles have been characterized by ultraviolet-visible spectroscopy and transmission electron microscopy, as a function of the ratio of silver ions to reducing agent molecules. The nanoparticle diameters are in the range of 2 to 40 nm. High-resolution transmission electron microscopy and fast Fourier transform analysis show that two kinds of crystal structures are obtained: face-centered cubic and hexagonal.
The synthesis of nanomaterials is of current interest due to their wide variety of applications in fields such as electronics[1–4], photonics[5–7], catalysis[8–10], medicine[11–15], etc. Most of the applications are due to the fact that matter at the nanometer scale has different properties as compared with the bulk state. For this reason, many research groups around the world are trying new methods of synthesis of different materials at the nanoscale. One goal is to control the size and shape of atomic clusters or nanoparticles and their ordering in 1D, 2D, or 3D arrays. In particular, silver nanoparticles have been used with promising results as bactericides[16–21], antimicotics, and anticancer agents[21, 23, 24].
Several methods have been devised in order to prepare metallic nanoparticles. For instance, one of the current methods crystalizes nanoparticles in microemulsions, using a variety of chemicals as precursors and large amounts of surfactants as stabilizing agents. The different preparation methods have been successful in the synthesis of nanoparticles of several materials: metallic[25–27], dielectric[28, 29], semiconductor[30, 31], and magnetic[32, 33]. However, the intensive use of solvents and synthetic reactants is harmful for the environment. For this reason, it is very desirable to devise alternative, ‘green’ methods of nanomaterial preparation that use environmentally friendly reactants. The silver nanoparticles obtained by the green synthesis method are candidates to be used in biological systems.
In the case of silver particles, the nanocrystals are usually grown from Ag+ solutions. The silver ions come from a salt like silver nitrate (AgNO3). The ions are first reduced to atoms by means of a reducing agent. The obtained atoms then nucleate in small clusters that grow into particles. Depending on the availability of atoms, which in turn depends on the silver salt to reducing agent concentration ratio, the size and shape of the nanoparticles can be controlled. In this method, two elements are needed for the nanoparticle grow: a silver salt and a reducing agent[34, 35].
On the other hand, in recent times, there is a growing interest in the synthesis of metal nanoparticles by ‘green’ methods. For this purpose, biomass or extracts of different plants have been tried with success as reducing agents. For instance, in the literature, there are reports of the synthesis of silver or gold nanoparticles using extracts of different plants[17–20, 23, 24, 36–49]. The present work is part of this new line of research.
In our study, the reducing agent comes from extracts of Rumex hymenosepalus, which is a plant rich in polyphenols. In the literature, there is no report on the synthesis of nanoparticles using extracts from this plant. It is a vegetal species abundantly present in North Mexico and in the south of the USA. In Mexico, it is collected, dried, cut, and packed for selling to the public. This plant, also known as canaigre dock or wild rhubarb, can be of interest for green synthesis because it contains a large amount of natural antioxidants. Among the antioxidant molecules this plant contains, polyphenolic compounds, like flavan-3-ols (tannins) and stilbenes, are found in large quantities. These molecules are potentially strong reducing agents due to their numerous OH groups that promote their antioxidant activity[50, 51].
In this paper, we present results on the synthesis of silver nanoparticles using extracts of the plant R. hymenosepalus (Rh extracts) as reducing agent in aqueous silver nitrate solutions. We have extracted the antioxidant fractions from dried roots of the plant. We have characterized the resulting nanoparticles by transmission electron microscopy (TEM) and ultraviolet-visible (UV-Vis) spectroscopy. To the best of our knowledge, this is the first report in the literature on nanoparticle synthesis using extracts of this plant.
We have purchased dried, slice-cut roots of R. hymenosepalus in a local convenient store (Comercial Zazueta, Hermosillo, Mexico); we present a picture of the dried roots in the Additional file1: Figure S1. Ethanol (99%) and silver nitrate (AgNO3 99%) are from Sigma-Aldrich (St. Louis, MO, USA). For the UV-Vis calibration curves, we have used epicatechin (98%) and epicatechin gallate (95%); both molecules were purchased in Sigma-Aldrich. We have used ultra-purified water (Milli Q system, Millipore, Billerica, MA, USA).
In order to prepare the plant extract, we have put 15 g of a dried R. hymenosepalus sample in a flask, and then, we have added 100 ml of an ethanol/water solution (70:30 v/v). The flask was stored at room temperature (T = 25°C), and the extraction was allowed to proceed during several days; the visual appearance of the liquid was monitored on a daily basis. Its color changed from a light red the first day to a darker brown. After 15 days, the extraction was considered complete since no change in the color was noticeable. The sample was then filtered, and the resulting liquid is the Rh (R. hymenosepalus) extract that has been used as reducing agent in the nanoparticle synthesis. The Rh extract has been characterized by UV-Vis spectroscopy (Perkin Elmer Lambda 20 spectrophotometer, PerkinElmer, Waltham, MA, USA) and proton nuclear magnetic resonance (1H NMR) experiments with a Bruker Avance 400 apparatus (Bruker AXS Inc., Madison, WI, USA) operating at 400 MHz, at 25°C. For the NMR experiments, a portion of the Rh extract was concentrated on a rotary evaporator at 37°C and dried under vacuum. The resulting dark brown solid was washed three times with 100 ml of tetrahydrofuran (Aldrich 99.9% purity) and purified using a glass filter. The filtrate was evaporated and dried under vacuum. With the solid, obtained NMR tubes were prepared in deuterated dimethyl sulfoxide (DMSO-d6). The internal reference was tetramethylsilane.
For the nanoparticle synthesis, we have prepared one solution of AgNO3 in water; the concentration was 0.1 M. Different volumes of this solution have been mixed with a fixed volume of the Rh extract (VRh = 200 μl); the total volume of each sample was adjusted to 4 ml by adding the necessary amount of ethanol in order to prepare samples with different AgNO3 concentrations: 2.5, 5, 7.5, 10, and 15 mM. The extract concentration was 5% v/v in all the samples.
For each AgNO3 concentration, the reduction reaction has proceeded along 96 h. The experiment was performed under regular, indoor illumination. The samples were analyzed every 24 h by visual inspection and UV-Vis spectroscopy. The nanoparticles have been observed with TEM using a Jeol 2010 F apparatus (JEOL Ltd., Akishima-shi, Japan) operating at 200 kV. We have deposited 10 μl of the nanoparticle suspension on a formvar-carbon coated copper TEM grid (300 Mesh). The sample was vacuum-dried for 24 h before observation. From the TEM micrographs, the size distribution was obtained, as well as the average diameter. The chemical composition of the nanoparticles has been obtained with energy dispersive X-ray spectroscopy (EDS) using a Bruker Quantax 200 detector (Bruker AXS Inc., Madison, WI, USA). The crystal structure of the nanoparticles has been obtained from high-resolution TEM (HR-TEM) experiments and from the corresponding fast Fourier transform (FFT) plots.
Results and discussion
The extraction procedure from dried R. hymenosepalus roots yielded a dark liquid which we examined spectroscopically. The UV-Vis and NMR experiments confirm the presence of polyphenols in the Rh extract. The absorbance of the sample presented a peak in the range of 277 to 280 nm as characteristic of polyphenol molecules. On the other hand, the 1H NMR proton spectra display a wealth of peaks characteristic of plant extracts (Additional file1: Figure S2). We have identified some of these signals as corresponding to polyphenol molecules (Additional file1: Figures S3 and S4). In particular, some peaks correspond to catechines and stilbene molecules. For instance, at least five chemical shifts of our spectra match those of epicatechin, as reported in the SDBS spectral database of organic compounds (no. 22007HSP-44-526). The coincidences are shown in Additional file1: Table S1. The chemical shifts also match those reported for epicatechin gallate and epigallocatechin gallate (Additional file1: Table S1). In the Additional file1: Figure S5, we display the chemical structure of these molecules. On the other hand, ten of the peaks match those reported for a stilbene compound extracted from roots of the Terminalia sericeae tree (Additional file1: Table S1). These signals correspond to a stilbene molecule known as stilbene glycoside (Additional file1: Figure S6). The NMR results obtained so far allow us to assess a significant presence of polyphenolic compounds in the plant extract of R. hymenosepalus. These compounds are potential reductor agents in the synthesis mechanism of silver nanoparticles. From UV-Vis calibration curves (using pure compounds), we estimate the concentration of two of the reducing molecules: epicatechin (241 μM) and epicatechin gallate (91.1 μM). Additional NMR experiments are under way in order to further characterize this plant extract. The results will be published elsewhere.
Since the R. hymenosepalus extract contains polyphenols, we can anticipate that it will serve as reducing agent for the nanoparticle synthesis. In fact, the same molecular mechanisms that give antioxidant properties to these molecules must promote the reduction of Ag+ ions to Ag atoms. The main mechanism is hydrogen abstraction due to the OH groups in the polyphenol molecules.
We have thus prepared silver nanoparticles using the R. hymenosepalus extracts as reducing agent. For all the AgNO3 concentrations, the samples changed their visual appearance shortly after addition of the plant extract, indicating that a reduction reaction took place. Initially, the reacting mixture was a slightly yellowish liquid; as the reaction proceeded, the solutions became orange, red, and brown. This is a strong indication of the formation of silver nanoparticles: the change in color is due to the strong absorption of visible light due to excitation of the nanoparticle surface plasmons[55–58]. In Figure 1, we show vials with reacting samples for different AgNO3 concentrations (0, 2.5, 5, 7.5, 10, and 15 mM), and different times after the reaction started (24, 48, 72, and 96 h); the clear time evolution is a signal of the growth of silver nanoparticles. The time scale of the visual evolution depends on the AgNO3 concentration. For instance, for CAgNO3 = 5 mM, nanoparticle formation is visually appreciable after 4 min of the beginning of the reaction.
The change in color, and thus the formation of silver nanoparticles, was confirmed by the UV-Vis experiments. In Figure 2, we show the spectra for a reaction time of 96 h. The curves display a pronounced peak around 425 nm, as expected from the plasmon resonance of silver nanoparticles. The UV-Vis peak is more pronounced for higher AgNO3 concentrations, indicating that more nanoparticles per unit volume are formed when this concentration increases. Note that in all the spectra displayed in Figure 2, the polyphenol peak (observed in the Rh extract) is also clearly visible around 278 nm. In the inset of Figure 2, we also display the UV-Vis spectra of the AgNO3 solution; it has a peak around 217 nm, as expected for Ag+ ions.
Note that we have performed control experiments in order to discard the action of ethanol and microorganisms as reducing agents. In the case of ethanol, the UV-Vis experiments show no significant Ag+ ions reduction when AgNO3 was dissolved, without Rh extract, in pure ethanol and in an ethanol/water mixture (see Additional file1: Table S2 and Figure S7). On the other hand, we have verified the absence of microorganisms in the samples. We have performed aerobic plate count experiments for mold, yeast, and aerobic mesophilic bacteria[59, 60], for the reacting sample where the silver nitrate concentration was 15 mM. In the case of the aerobic mesophilic bacteria test, we used plate count agar as culture medium; the sample was incubated at 35°C for 48 h. The results show that no mesophilic bacteria grow in the plate (see Additional file1: Figure S8). In fact, the colony forming unit (CFU) is <1 CFU/ml. For the mold and yeast count test, we used potato dextrose agar; the sample was incubated at 25°C for 5 days. No mold or yeast was detected in the plate (the resulting CFU is <1 CFU/ml) (see Additional file1: Figure S8). Thus, we can be sure that the synthesis of nanoparticles was performed under fully abiotic conditions.
We have characterized the silver nanoparticles with transmission electron microscopy. The size and abundance of the resulting particles depend on the AgNO3 concentration. Their diameter is in the range of 2 to 40 nm. In Figures 3 and4, we present micrographs of the obtained silver nanoparticles after 24 and 96 h of the beginning of the reaction, for the different AgNO3 concentrations. For a reacting time of 24 h (Figure 3), we can appreciate that for CAgNO3 = 2.5 mM (micrograph A), the population is composed mainly of scattered, small nanoparticles. As the CAgNO3 increases, bigger nanoparticles are observed, while the proportion of small nanoparticles decreases. This trend is somehow maintained for a reacting time of 96 h (Figure 4). From the micrographs, we can observe that a population of big nanoparticles, in coexistence with a small proportion of small particles, is clearly appreciated. Furthermore, the size of the bigger particles increases as CAgNO3 is increased, while at the same time, the proportion of small nanoparticle decreases. Note that we do not observe particle coalescence, probably due to a stabilizing effect produced by the antioxidant molecules.
We have quantified these tendencies by statistically analyzing a population of more than 500 nanoparticles for each reaction time. The results are shown in Figure 5, where for matters of clarity, we present the full histograms for 96 h of reaction time, and only a representative curve for 24 h. For the shorter reaction time (24 h, black curves in Figure 5), most of the particles are small, with an average diameter around 3 to 5 nm. For 96 h after the beginning of the reaction, two populations are clearly distinguishable in the histograms. The first one is a subpopulation of small nanoparticles of average diameter around 4 to 5 nm. However, there exists also a considerable fraction of nanoparticles with larger average diameters, of the order of 10 to 20 nm. The average diameter of these larger particles grows with an increase in the AgNO3 concentration.
The chemical composition of the obtained particles was assessed by EDS spectroscopy. From the EDS spectra (see Additional file1: Figure S9), we have confirmed that the nanoparticles are mainly composed of silver (subtracting the Cu, Si, and C contributions from the TEM grid and the detector window). Some amount of oxygen is also displayed in the EDS results (see Additional file1: Table S3), probably meaning that some trace amount of the extract is still present in the TEM grid.
The crystallographic analysis confirms that the nanoparticles are indeed silver crystals. For instance, in Figures 6 and7, we show HR-TEM images of two representative nanoparticles, with the corresponding FFT plot. Very interestingly, these results show that the nanoparticle population has a combination of two kinds of crystal symmetries: face centered cubic (fcc) and hexagonal (4H). The prevalence rates of these geometries are 79% (fcc) and 21% (4H). We have computed the interplanar distances from the micrographs and the FFT plots. In the case of the fcc nanoparticles, the interplanar distances are d1 = 2.316 Å, d2 = 1.517 Å, and d3 = 1.159 Å. They are, respectively, associated with the planes (111), (220), and (222) corresponding to the fcc structure of a silver crystal. On the other hand, the interplanar distances for the 4H structure are d1 = 2.405 Å, d2 = 2.275 Å, d3 = 1.407 Å, d4 = 1.249 Å, and d5 = 1.149 Å, corresponding to the planes (101), (1-12), (110), (008), and (203) of a hexagonal 4H structure. We have characterized the nanoparticle population for both the fcc and 4H structures, analyzing 100 particles. The results are shown in Figure 8. We observe that the fcc nanoparticles display two size populations: one with a small average diameter (around 10 nm) and a second one with a larger diameter (around 28 nm). On the other hand, the hexagonal nanoparticles have only one size population and larger diameters (around 38 nm). Note that the results shown in Figure 8 correspond to samples where the reaction time is of 30 days.
The observed features in the TEM, UV-Vis,, and visual observation experiments can be summarized and understood as follows. The polyphenols contained in the R. hymenosepalus extracts act effectively as reducing agents for the Ag+ ions due to their antioxidant activity. The reduction reaction promotes the nucleation and growth of nearly spherical Ag nanoparticles. As expected, the kinetics of nanoparticle formation, as well as the resulting nanoparticle populations, depends on the AgNO3 concentration. Higher silver nitrate concentrations yield more nanoparticles for reacting times of 24 h, because more material is available for the nanoparticle growth. However, when the reaction time is 96 h, two populations of nanoparticles are present. In this case, most of the silver atoms are accommodated in large nanoparticles.
We have prepared silver nanoparticles using extracts of R. hymenosepalus, a plant abundantly found in North Mexico and in the south of the USA, as reducing agent. The results are very promising since the extract promotes the formation of nanoparticles at room temperature with a fast kinetics and with no harmful chemicals. Our method is easy to perform in a single step. NMR and UV-Vis spectroscopy experiments show that R. hymenosepalus is a plant rich in polyphenols, such as catechines and stilbenes, molecules that have antioxidant activity and are also found in plants like green tea and grapes. The same molecular mechanisms responsible of the antioxidant activity allow the use of these molecules as reducing agents and stabilizing effects for silver nanoparticles. The silver nanoparticles synthesized by this method are strong candidates for its use in biological systems. The diameter of the silver nanoparticles is in the range of 2 to 40 nm, as shown by TEM experiments. Interestingly, the silver nanoparticle population is composed of a mixture of face-centered cubic and hexagonal structures. The presence of the hexagonal crystal atypical structure 4H for silver nanoparticles was obtained by this method, opening a new route to study catalytical activity, antimicrobial properties, and the optical response of this nanomaterial.
Lu W, Lieber CM: Nanoelectronics from the bottom up. Nat Mater 2007, 6: 841–850. 10.1038/nmat2028
Lugli P, Locci S, Erlen C, Csaba G: Nanotechnology for Electronics, Photonics, and Renewable Energy Molecular Electronics: Chapter 1 Challenges and Perspectives. Edited by: Korkin A, Krstic PS, Wells JC. New York: Springer; 2010:1–40.
Karni TC, Langer R, Kohane DS: The smartest materials: the future of nanoelectronics in medicine. ACS Nano 2012, 6: 6541–6545. 10.1021/nn302915s
Mitin VV, Kochelap VA, Stroscio MA (Eds): Introduction to nanoelectronics: materials for nanoelectronics. UK: Cambridge; 2008:65–108.
Shen Y, Friend CS, Jiang Y, Jakubczyk D, Swiatkiewicz J, Prasad PN: Nanophotonics: interactions, materials, and applications. J Phys Chem B 2000, 104: 7577–7587. 10.1021/jp0016131
Zalevsky Z, Mico V, Garcia J: Nanophotonics for optical super resolution from an information theoretical perspective: a review. Journal of Nanophotonics 2009, 3: 1–18.
Taylor A (Ed): Nanophotonics: Nanoscale Phenomena Underpinning Nanophotonics. Washington: The National Academies Press; 2008:19–82.
Kalidindi SB, Jagirdar BR: Nanocatalysis and prospects of green chemistry. Chem Sus Chem 2012, 5: 65–75.
Serp P, Philippot K: Nanomaterials in Catalysis: Concepts in Nanocatalysis. Edited by: Serp P, Philippot K. Weinheim: Wiley-VCH Verlag; 2013:1–54.
Kung HH, Kung MC: Nanotechnology in Catalysis Vol. 3: Nanotechnology and Heterogeneous Catalysis. Edited by: Zhou B, Han S, Raja R, Somorjai GA. New York: Springer; 2007:1–11.
Shomura Y: Advances in Composite Materials for Medicine and Nanotechnology: Composite Material Stent Comprising Metallic and Non-metallic Materials. Edited by: Attaf B. Croatia: InTech; 2011:59–74.
Jotterand F, Alexander AA: Biomedical Nanotechnology: Managing the “Known Unknowns”: Theranostic Cancer Nanomedicine and Informed Consent. Edited by: Hurst SJ. Illinois: Springer; 2011:413–430.
Lee H, Messersmith PB: Nanotechnology in Biology and Medicine: Bio-Inspired Nanomaterials for a New Generation of Medicine. Edited by: Vo-Dinh T. Florida: Taylor and Francis; 2007:1–9.
Etheridge ML, Campbell SA, Erdman AG, Haynes CL, Wolf SM, McCullough J: The big picture on nanomedicine: the state of investigational and approved nanomedicine products. Nanomedicine: Nanotechnology, Biology, and Medicine 2013, 9: 1–14. 10.1016/j.nano.2012.05.013
Mata A, Palmer L, Tejeda-Montes E, Stupp SI: Nanotechnology in Regenerative Medicine: Chapter 3 Design of Biomolecules for Nanoengineered Biomaterials for Regenerative Medicine. Edited by: Navarro M, Planell JA. Barcelona: Springer; 2012:39–49.
Zhang H, Wu M, Sen A: Nano-Antimicrobials: Silver Nanoparticle Antimicrobials and Related Materials. Edited by: Cioffi N, Rai M. New York: Springer; 2012:3–45.
Niraimathi KL, Sudha V, Lavanya R, Brindha P: Biosynthesis of silver nanoparticles using Alternanthera sessilis (Linn.) extract and their antimicrobial, antioxidant activities. Colloids Surf B Biointerfaces 2013, 102: 288–291.
Sharma VK, Yngard RA, Lin Y: Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 2009, 145: 83–96. 10.1016/j.cis.2008.09.002
Vankar PS, Shukla D: Biosynthesis of silver nanoparticles using lemon leaves extract and its application for antimicrobial finish on fabric. Appl Nanosci 2012, 2: 163–168. 10.1007/s13204-011-0051-y
Narayanan KB, Sakthivel N: Green synthesis of biogenic metal nanoparticles by terrestrial and aquatic phototrophic and heterotrophic eukaryotes and biocompatible agents. Adv Colloid Interface Sci 2011, 169: 59–79. 10.1016/j.cis.2011.08.004
Ashanrani PV, Kah Mun GL, Hande MP, Valiyaveettil S: Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 2009, 3: 279–290. 10.1021/nn800596w
Panacek A, Kolar M, Vecerova R, Prucek R, Soukupová J, Krystof V, Hamal P, Zboril R, Kvítek L: Antifungal activity of silver nanoparticles against Candida spp. Biomaterials 2009, 30: 6333–6340. 10.1016/j.biomaterials.2009.07.065
Singh S, Saikia JP, Buragohain AK: A novel ‘green’ synthesis of colloidal silver nanoparticles (SNP) using Dillenia indica fruit extract. Colloids Surf B Biointerfaces 2013, 102: 83–85.
Das S, Das J, Samadder A, Bhattacharyya SS, Das D, Khuda-Bukhsh AR: Biosynthesized silver nanoparticles by ethanolic extracts of Phytolacca decandra , Gelsemium sempervirens , Hydrastis canadensis and Thuja occidentalis induce differential cytotoxicity through G2/M arrest in A375 cells. Colloids Surf B Biointerfaces 2013, 101: 325–336.
Murphy CJ, Sau TK, Gole AM, Orendorff CJ, Gao J, Gou L, Hunyadi SE, Li T: Anisotropic metal nanoparticles: synthesis, assembly, and optical applications. J Phys Chem B 2005, 109: 13857–13870. 10.1021/jp0516846
Ledwith DM, Aherne D, Kelly JM: Metallic Nanomaterials Vol. 1: Approaches to the Synthesis and Characterization of Spherical and Anisotropic Silver Nanomaterials. Edited by: Kumar SSR. Weinheim: Wiley-VCH Verlag; 2009:99–148.
Yu CH, Tam K, Tsang ESC: Handbook of Metal Physics, Vol. 5: Chapter 5 Chemical Methods for Preparation of Nanoparticles in Solution. Edited by: Blackman J. Amsterdam: Elsevier; 2009:113–141.
Nelson JK: Overview of nanodielectrics: insulating materials of the future. In Proceedings of Electrical Insulation Conference and Electrical Manufacturing Expo, October 2007. Nashville: EEIC; 2007:229.
Baklanov MR: Nanodevices and Nanomaterials for Ecological Security: Chapter 1 Nanoporous Dielectric Materials for Advanced Micro- and Nanoelectronics. Edited by: Shunin YN, Kiv AE. The Netherlands: Springer; 2012:3–18.
Zhang JZ, Wang Z, Liu J, Chen S, Liu G: Self-Assembled Nanostructures: Chapter 8 Optical, Electronic, and Dynamic Properties of Semiconductor Nanomaterials. Edited by: Lockwood DJ. Ontario: Kluwer Academic Publishers; 2004:201–255.
Weber C, Richter M, Ritter S, Knorr A: Semiconductor Nanostructures: Chapter 9 Theory of the Optical Response of Single and Coupled Semiconductor Quantum Dots. Edited by: Bimberg D. Berlin: Springer; 2008:189–210.
Lu A, Salabas EL, Schüth F: Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew Chem Int Ed 2007, 46: 1222–1244. 10.1002/anie.200602866
Koksharov YA: Magnetic Nanoparticles: Magnetism of nanoparticles: effects of size, shape and interactions. Edited by: Gubin SP. Moscow: Wiley-VCH Verlag; 2009:117–196.
Durán N, Marcato PD: Nano-Antimicrobials: Chapter 12 Biotechnological Routes to Metallic Nanoparticles Production: Mechanistic Aspects, Antimicrobial Activity, Toxicity and Industrial Applications. Edited by: Cioffi N, Rai M. Berlin Heidelberg: Springer-Verlag; 2012:337–374.
Prabhu S, Poulose EK: Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Nano Lett 2012, 2: 1–10. 10.1186/2228-5326-2-1
Suriyakalaa U, Antony JJ, Suganya S, Siva D, Sukirtha R, Kamalakkannan S, Pichiah PB, Achiraman S: Hepatocurative activity of biosynthesized silver nanoparticles fabricated using Andrographis paniculata . Colloids Surf B Biointerfaces 2013, 102: 189–194.
Mohanpuria P, Rana NK, Yadav SK: Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 2008, 10: 507–517. 10.1007/s11051-007-9275-x
Gardea-Torresdey JL, Parsons JG, Gomez E, Peralta-Videa J, Troiani HE, Santiago P, Yacaman MJ: Formation and growth of Au nanoparticles inside live alfalfa plants. Nano Lett 2002, 2: 397–401. 10.1021/nl015673+
Armendariz V, Herrera I, Peralta-Videa JR, Yacaman MJ, Troiani H, Santiago P, Gardea-Torresdey JL: Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nanobiotechnology. Journal of Nanoparticle Research 2004, 6: 377–382. 10.1007/s11051-004-0741-4
Kumar VG, Gokavarapu SD, Rajeswari A, Dhas TS, Karthick V, Kapadia Z, Shrestha T, Barathy IA, Roy A, Sinha S: Facile green synthesis of gold nanoparticles using leaf extract of antidiabetic potent Cassia auriculata . Colloids Surf B Biointerfaces 2011, 87: 159–163. 10.1016/j.colsurfb.2011.05.016
Ghoreishi SM, Behpour M, Khayatkashani M: Green synthesis of silver and gold nanoparticles using Rosa damascena and its primary application in electrochemistry. Physica E 2011, 44: 97–104. 10.1016/j.physe.2011.07.008
Dubey SP, Lahtinen M, Sillanpää M: Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa . Colloids and Surfaces A: Physicochem Eng Aspects 2010, 364: 34–41. 10.1016/j.colsurfa.2010.04.023
Dubey SP, Lahtinen M, Sillanpää M: Tansy fruit mediated greener synthesis of silver and gold nanoparticles. Process Biochem 2010, 45: 1065–1071. 10.1016/j.procbio.2010.03.024
Shankar SS, Ahmad A, Sastry M: Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol Prog 2003, 19: 1627–1631. 10.1021/bp034070w
Shankar SS, Rai A, Ahmad A, Sastry M: Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using Neem ( Azadirachta indica ) leaf broth. J Colloid Interface Sci 2004, 275: 496–502. 10.1016/j.jcis.2004.03.003
Parsons JG, Peralta-Videa JR, Gardea-Torresdey JL: Developments in Environmental Science, Volume 5: Chapter 21 Use of plants in biotechnology: Synthesis of metal nanoparticles by inactivated plant tissues, plant extracts, and living plants. Edited by: Sarkar D, Datta R, Hannigan R. Minessota: Elsevier; 2007:436–485.
Dubey SP, Lahtinen M, Särkkä H, Sillanpää M: Bioprospective of Sorbus aucuparia leaf extract in development of silver and gold nanocolloids. Colloids Surf B Biointerfaces 2010, 80: 26–33. 10.1016/j.colsurfb.2010.05.024
Noruzi M, Zare D, Davoodi D: A rapid biosynthesis route for the preparation of gold nanoparticles by aqueous extract of cypress leaves at room temperature. Spectrochimica Acta Part A 2012, 94: 84–88.
Narayanan KB, Sakthivel N: Coriander leaf mediated biosynthesis of gold nanoparticles. Mater Lett 2008, 62: 4588–4590. 10.1016/j.matlet.2008.08.044
Gan PP, Yau Li SF: Potential of plant as a biological factory to synthesize gold and silver nanoparticles and their applications. Rev Environ Sci Biotechnol 2012, 11: 169–206.
VanderJagt TJ, Ghattas R, VanderJagt DJ, Crossey M, Glew RH: Comparison of the total antioxidant content of 30 widely used medicinal plants of New Mexico. Life Sci 2002, 70: 1035–1040. 10.1016/S0024-3205(01)01481-3
Navarro RE, Santacruz H, Inoue M: Complexation of epigallocatechin gallate (a green tea extract, egcg) with Mn2+: nuclear spin relaxation by the paramagnetic ion. J Inorg Biochem 2005, 99: 584–588. 10.1016/j.jinorgbio.2004.11.013
Joseph CC, Moshi MJ, Innocent E, Nkunya MHH: Isolation of a stilbene glycoside and other constituents of Terminalia sericeae . Afr J Tradit Complementary and Alternative Medicine 2007, 4: 383–386.
Sivaraman SK, Elango I, Kumar S, Santhanam V: A green protocol for room temperature synthesis of silver nanoparticles in seconds. Curr Sci 2009, 97: 1055–1059.
Kwon MJ, Lee J, Wark AW, Lee HJ: Nanoparticle-enhanced surface plasmon resonance detection of proteins at attomolar concentrations: comparing different nanoparticle shapes and sizes. Anal Chem 2012, 84: 1702–1707. 10.1021/ac202957h
Ray PC: Size and shape dependent second order nonlinear optical properties of nanomaterials and their application in biological and chemical sensing. Chem Rev 2010, 110: 5332–5365. 10.1021/cr900335q
Amendola V, Bakr OM, Stellacci F: A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: effect of shape, size, structure, and assembly. Plasmonics 2010, 5: 85–97. 10.1007/s11468-009-9120-4
Hartland GV: Optical studies of dynamics in noble metal nanostructures. Chem Rev 2011, 111: 3858–3887. 10.1021/cr1002547
Maturin L, Peeler JT: Chapter 3: Aerobic Plate Count. Bacteriological Analytical Manual. Silver Spring, MD: U.S. Food and Drug Administration; 1998.
Tournas VH, Katsoudas E, Miracco EJ: Moulds, yeasts and aerobic plate counts in ginseng supplements. Int J Food Microbiol 2006, 108: 178–181. 10.1016/j.ijfoodmicro.2005.11.009
Joint Committee on Powder Diffraction Standards: Powder Diffraction File Card 04–0783. Swathmore, PA: International Center for Diffraction Data; 1987.
This research was partially funded by Consejo Nacional de Ciencia y Tecnología (Conacyt - Mexico): grants 128192 and 105236. ERL acknowledges a graduate grant from Conacyt. The TEM experiments were performed in the Laboratorio de Microscopía Electrónica de la Universidad de Sonora.
The authors declare that they have no competing interests.
ERL, RIP, and REN carried out the experiments. ERL, RIP, REN, JT, RHU, and AM analyzed the data. CIP conducted the plate count experiments. ERL, RIP, JT, and AM developed the conceptual framework, and AM supervised the whole work. ERL, RIP, and AM drafted the paper. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: Dried roots of Rumex hymenosepalus (Figure S1).1H NMR spectra of Rh in DMSO-d6 referenced to TMS (Figure S2). Section of the 1H NMR spectra of the Rh extract (Figure S3). Following section of the 1H NMR spectra of the Rh extract (Figure S4). 1H NMR chemical shifts for the Rh extract (first column) as compared to those reported in the literature (Table S1). Molecular structure of the catechin compounds found in the Rh extract (Figure S5). Molecular structure of stilbene glycoside found in the Rh extract (Figure S6). Composition of samples without Rh extract (Table S2). UV-Vis spectrum of solutions without Rh extract (Figure S7). Sterility test of (A) aerobic mesophilic bacteria and (B) mold and yeast (Figure S8). EDS spectra for a silver nanoparticle (Figure S9). Chemical analysis of the EDS results for a silver nanoparticle (Table S3). (PDF 768 KB)
Authors’ original submitted files for images
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