Table 1 Comparison of different immunoassay methods used for protein detection
From: Protein detection through different platforms of immuno-loop-mediated isothermal amplification
Method | Required time | Complicacy | Requirement of high expertise and instrumentation | Cost | Specificity | Sensitivity | Quantification capacity | Potential of being as high-throughput test | Potential for point-of-care application | Risk of cross-contamination |
---|---|---|---|---|---|---|---|---|---|---|
iLAMP | Low | Low | No | Low | High | High | Yes | Yes | Yes | Low |
iPCR | High | High | Yes | High | Intermediate-high | Intermediate-high | No | No | No | High |
iReal-time PCR | High | High | Yes | High | Intermediate-high | Intermediate-high | Yes | No | No | High |
iRCA | High | High | Yes | High | High | Intermediate-high | Yes | Yes | No | High |
iNASBA | High | High | Yes | High | High | Intermediate-high | Yes | Yes | No | High |
ELISA | High | High | Yes | High | Low | Low | Yes | Yes | No | High |