Figure 8

Agarose gel electrophoresis of plasmid pZPY112 treated with different concentrations of the silver nanoparticles (μg/100 μl). Lane 1, DNA molecular weight marker. Lane 2, control plasmid without silver nanoparticles showing only supercoiled plasmid band that moves ahead of relaxed circular and linear plasmids. Lane 3, plasmid incubated with 0.51 μg nanoparticles showing disappearance of the supercoiled plasmid band and appearance of relaxed circular and linear plasmid bands along with smaller fragmented DNA. Lane 4, plasmid incubated with 1.02 μg nanoparticles. Lane 5, plasmid incubated with 2.55 μg nanoparticles. Lane 6, plasmid incubated with 3.57 μg nanoparticles showing gradual degradation of the fragmented DNA bands; and lane 7, plasmid incubated with 5.1 μg nanoparticles showing more degradation of DNA.