Size- and time-dependent alteration in metabolic activities of human hepatic cytochrome P450 isozymes by gold nanoparticles via microsomal coincubations

Table 1 Summary of accuracy and precision of the analytical method for the six probe CYP metabolites

Analyte	Enzyme	QC concentration (μM)			Mean accuracy (%)			Mean precision (RSD%)
Analyte	Enzyme	Low	Mid	High	Low	Mid	High	Low	Mid	High
Acetaminophen	CYP1A2	0.076	7.6	152.1	103	104	90	7.86	4.20	8.57
Hydroxy tolbutamide	CYP2C9	0.035	3.5	69.8	111	107	91	2.48	1.13	2.39
4-Hydroxymephenytoin	CYP2C19	0.043	4.3	85.38	103	102	112	6.35	2.73	3.23
Dextrorphan	CYP2D6	0.026	2.6	51.0	109	98	88	4.55	4.67	5.14
6β-Hydroxytestosterone	CYP3A4	0.033	3.3	65.7	98	110	93	10.28	4.75	7.27
Dehydro felodipine	CYP3A4	0.026	2.6	52.3	104	97	110	5.53	6.67	2.67