- Nano Express
- Open Access
Nano- and microsized zeolites as a perspective material for potentiometric biosensors creation
© Soldatkin et al.; licensee Springer. 2015
- Received: 12 November 2014
- Accepted: 19 January 2015
- Published: 11 February 2015
A number of potentiometric biosensors based on coimmobilization of enzymes with different types of zeolite on pH-ion-sensitive field-effect transistor (ISFET) have been developed. Their working characteristics have been determined and compared.
It was shown that clinoptilolite and zeolite Beta polymorph A (BEA) are more promising for creating biosensors than zeolite A. Changing the concentration of zeolite BEA in membranes, it is possible to extend the biosensor linear measurement range. The two-layer method of deposition of the enzyme with clinoptilolite was found to provide a significant increase in the biosensor sensitivity to substrates, whereas thermal modification of the zeolite BEA crystals can improve analytical characteristics of potentiometric biosensors for detection of toxic substances.
These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via different zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.
- Zeolite BEA
- Zeolite A
Immobilization of biological material is a problem of prime importance for the biosensor creation. Successful immobilization directly influences key characteristics of biosensors (activity of biological material, linear working range, sensitivity to substrate, reproducibility and operational stability of signals, duration of storage, etc.). Therefore, selection of main parameters of the immobilization process is an essential challenge in the development of biosensors. In particular, an improvement of the methods of biomembrane immobilization onto the sensing element is an important task. One of the trends for solving this problem is an application of adjuvant substances (that are carriers of biomaterial, stabilizing agents, mediators, etc.) during immobilization process.
In the literature, there are various reports about physical adsorption of enzyme on the transducer solid substrates with some supporting materials such as sol-gels [1,2], polymeric membranes [3,4] and microcapsules [5,6], nanotubes [7,8], and zeolites [9-12]. The goal of this work was to study the controlled influence of zeolite crystals on the function of potentiometric biosensors.
The use of zeolites in combination with enzymes is of considerable interest due to such characteristics of zeolites as an ability to change the surface groups, hydrophilic/hydrophobic properties, geometry, surface charge, crystal and pore sizes, and capability of acidity regulation. Additionally, zeolites are stable at high temperatures, insoluble in organic solvents, and resistant to harsh conditions of the experiment. Thus, zeolites can be used to control the microenvironment of enzymes . Accordingly, the potential advantages of zeolite integration can be studied at the development of novel biosensors with improved analytical characteristics using coimmobilization of enzymes and zeolites on the surface of relevant transducers.
Zeolites can be embedded into the bioselective elements to improve analytical characteristics of biosensors, i.e., their sensitivity to the substrate, linear and dynamic ranges, and inter- and intra-reproducibility of signals. As shown in [14,15], zeolites of various kinds can be effectively used for the glucose oxidase immobilization when developing glucose amperometric biosensors to optimize their sensitivity, selectivity, and stability. As reported in [10,16,17], zeolites are used as alternative carriers for the enzyme immobilization at the creation of conductometric biosensors. Diverse variants of coimmobilization of the enzyme onto the surface of conductometric transducers were analyzed with respect to the improvement of analytical characteristics of biosensors.
In this paper, we studied and analyzed the use of a number of zeolites to improve performance characteristics of the biosensors based on pH-ion-sensitive field-effect transistor (ISFET).
In the work, the following enzymes were used for biosensor creation: urease (EC 184.108.40.206) from Canavalia ensiformis, activity 66.3 U/mg (Fluka, Switzerland) and activity 22 U/mg and 35 U/mg (Sigma, Switzerland) and butyrylcholinesterase (EC 220.127.116.11) from equine serum, activity 13 U/mg and activity 20 U/mg (Sigma, Germany). Glycerol, bovine serum albumin (BSA; fraction V), 50% aqueous solution of glutaraldehyde (GA), sodium and potassium chlorides, urea, and butyrylcholine chloride were from Sigma-Aldrich Chemie (Steinheim, Germany). Potassium-phosphate buffer (KH2PO4-K2HPO4) and NaOH were produced by Helicon (Moscow, Russia) and Sigma-Aldrich Chemie (Steinheim, Germany). Glycoalkaloids α-chaconine (purity 95%) and α-solanine (purity 95%) from potato sprouts were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and used as inhibitors for BuChE. Mercury nitrate (Hg(NO3)2) from Himlaborreaktiv (Kiev, Ukraine) was used as an activity inhibitor for urease. Other inorganic substances were of analytical grade (>98%).
Synthesis and ion exchange procedure of zeolite crystals
Zeolite Na+-BEA samples were hydrothermally synthesized from gel solutions having the compositions of 1,92Na2O:Al2O3:ySiO2:4,6(TEA)2O:444H2O. The composition was determined according to the changing SiO2/Al2O3 ratio (y = 30, 40, 50, and 60) of the Na+-BEA samples. Sodium aluminate precursor solution for zeolite Beta polymorph A (BEA) was prepared by dissolving NaOH (>97 wt%, J.T.Baker) and sodium aluminate (50.8 wt% Al2O3 and 43.4 wt% Na2O, Riedel-de Haën) in deionized water (resistivity > 18 MΩ cm). Then, tetraethyl ammonium hydroxide solution (TEAOH; 35 wt%, in water, Sigma-Aldrich, Steinheim, Germany), which is the structure directing agent for zeolite BEA synthesis, was added, and the prepared precursor solution was stirred thoroughly. LUDOX® HS-40 colloidal silica solution (40 wt% SiO2 suspension in water, Sigma Aldrich, Steinheim, Germany) was added into aluminate precursor and mixed thoroughly before putting into the Teflon-lined stainless steel autoclaves. The autoclaves were kept statically at 120°C in a conventional oven for 7 days. The resulting solid particles were vacuum-filtered, washed with deionized water, and dried at room temperature.
The NH4 + forms of BEA crystals were obtained by ion-exchange with 1 M aqueous ammonium nitrate at 80°C for 24 h. The acid (H+) forms were made by calcination of NH4 +-BEA 30 crystals at 500°C for 6 h.
Molar compositions, synthesis conditions, Si/Al ratio, and particle diameters of zeolite samples
Si/Al ratio (crystal)a
Particle size (μm)b
120°C, 7 days
9.7 ± 1.0
120°C, 7 days
13.5 ± 1.4
120°C, 7 days
17.7 ± 1.8
100°C, 2 days
100°C, 3 days
100°C, 5 days
The powdered sample of clinoptilolite with unit cell formula (Na0.10 K0.57)(Ca0.47 Mg0.15)(Al1.97Fe0.12)(Si9.96Ti0.02)O24 × 7H2O and the average particle size of 0.4 μm was obtained from Société Méditerranéenne des Zéolithes (Montpellier, France).
Characterization of zeolite
The phase identification was accomplished using X-ray powder diffraction (XRD; Philips PW 1729 diffractometer, Ni-filtered Cu Kα radiation, Philips Electronics, Almelo, Netherlands). Crystal morphology/texture/surface features were examined by field emission scanning electron microscopy (FE-SEM; FEI 400 Quanta, FEI, Hillsboro, USA). Energy dispersive X-ray spectroscopy (EDX; Phoenix EDAX X-ray analyzer equipped with a Sapphire super ultra-thin window detector attached to a Hitachi S-4700 FE-SEM) was used to determine the crystal Si/Al ratios. Nitrogen adsorption-desorption isotherms were measured at the liquid nitrogen temperature using a Quantachrome Autosorb-6 analyzer (Quantachrome Instruments, Boynton Beach, USA). Prior to the measurements, samples were dried at 110°C for 12 h and outgassed at 300°C under high vacuum for 3 h. Multi-point Brunauer-Emmett-Teller (BET) specific surface areas (S BET) were calculated in the P/P o range 0.05 to 0.30. The external specific surface areas (S ext) were estimated from the slope of the second linear segments on de Boer's adsorbate thickness t-plots. Saito-Foley (SF) method was applied for the pore volume and pore size analysis. The zeta potential of all samples was obtained by a zeta potential measurement system (Nano ZS90, Malvern Instruments, Worcestershire, UK) at 25°C. The particles were dispersed in deionized water (solid load is 1 wt%) and ultrasonicated for 1 h. After the ultrasonication, zeta potential was measured as a function of pH by titration with 0.1 M HCl or 0.1 M NaOH. The particle size distributions were measured on API Aerosizer LD equipped with API Aero-Disperser dry powder dispersion system (TSI, Inc., Shoreview, USA; Particle Instruments/Amherst, Amherst, USA). For these measurements, the density of zeolite Beta was assumed to be 1.61 g cm−3 . Diffuse reflectance infrared Fourier transform (DRIFT) spectra were acquired on a Nicolet Magna-IR 560 spectrometer (Thermo Nicolet Corp., Madison, USA) supplied with a DTGS KBr detector (Thermo Nicolet Corp., Madison, USA) and a Spectra-Tech (Spectra-Tech, Inc., Oak Ridge, USA) diffuse reflectance high-temperature/vacuum chamber with KBr windows. The chamber loaded with crystals (0.02 g) was heated to 350°C for 2 h to dehydrate the samples. Subsequently, the spectra were collected 10 min after the samples were cooled to 100°C. The samples were under dry nitrogen (99.9% purity with <10 ppm moisture content, Med-Tech Gases, North Billerica, USA) flowing at 33 mL min−1 (STP) during the heat treatment in the chamber and during spectra acquisition. Before collection of the DRIFT spectrum, nitrogen was purged through the beam path at 14 L min−1 (NTP). The spectra were collected with resolution of 2 cm−1 using 128 scans. Potassium bromide (99 + % KBr, infrared grade, Acros Organics, Morris Plains, USA) was used as the background, and the samples were analyzed neat.
Properties of zeolite samples
Surface area (m 2 /g)b
Pore size (nm)с
Pore volume (cc/g)c
Particle size (μm)
Na + -BEA30
10 ± 1.0
NH4 + -BEA30
10 ± 1.0
H + -BEA30
10 ± 1.0
Na + -BEA50
17.7 ± 1.8
NH4 + -BEA50
17.7 ± 1.8
H + -BEA50
17.7 ± 1.8
Linde type A (LTA) samples had almost the same surface area (500 to 550 m2/g) but differed in pore size, pore volume, and particle size. Si/Al ratio of all the LTA zeolites during synthesis was 0.84.
Heat treatment of zeolite
Heat treatment procedures used to prepare different zeolite Beta samples
Final temperature (°C)
Duration at final temperature (h)
Heating rate (°C min −1 )
ISFETs were fabricated at the Institute of Semiconductor Physics (Kiev, Ukraine). Potentiometric sensor chip contains two identical p-channel transistors on the same crystal of total area 8 × 8 mm2. The diffusion р + -buses with contacts to the drain and source of each transistor are coming out on the edge of the chip along with an outlet to the built-in reference microelectrode. To prevent the formation of a parasitic conductivity channel between p + -areas of two transistors, the chip has a 50 μm wide protecting division n + -line with a contact to the substrate. Zigzag geometry of the transistor gate area with the width-to-length ratio 100:1 ensures sufficient steep slope of the transfer characteristic. The window etched in an oxide layer for growing gate dielectric replicates the channel geometry with a 7 μm overlapping.
The pH-FETs responses were measured using the circuit maintaining constant value of the current in the transistor channel, enabling the output signal to follow automatically the changes in potential value near the transistor gate. The threshold voltage for all pH-FETs was about −2.5 V. The measurements were performed under the following conditions: channel current of approximately 200 μA, drain-source voltage close to 1 V, and the sensor bulk is connected to the circuit ground. Transconductance of the used ISFETs measured in phosphate buffer with Ag/AgCl reference electrode was in the range of 300 to 400 μA/V. Typical current vs. pH response measured with standard buffer solutions (pH 4.0, 7.0, and 9.0) was in the range of 15 to 20 μA/pH, which gives us primary pH sensitivity estimation of approximately 50 mV/pH.
Preparation of bioselective membranes
To produce a working bioselective membrane based on urease and zeolites, two mixtures were prepared. The first one contained 10% enzyme, 10% BSА, 10% glycerol in 20 mM phosphate buffer, pH 7.2, and the second - zeolite of different weight concentrations in 20 mM phosphate buffer, pH 7.2, with 10% glycerol. Prior to deposition on the transducer surface, the two mixtures were mixed in equal volumes. The final mixture was immediately deposited on the transducer surfaces using the Eppendorf microsampler (with variable volume 0.1 to 2.5 μL) till complete coverage of the working surfaces. The volume of each membrane was about 0.05 μL. The reference membrane mixture contained 10% BSA and 10% glycerol in 20 mM phosphate buffer, pH 7.2. All the membrane mixtures contained the same total amount of protein. Then, the transducers with deposited membrane mixtures were placed into glutaraldehyde saturated vapor for 15 to 25 min; afterwards, they were dried in the air at room temperature for 15 min and washed in the buffer solution from excess unbound proteins, zeolites, and GA.
Measurements were carried out at room temperature in a working cell filled with 5 mM phosphate buffer, pH 7.2. The substrate concentrations were varied by addition of different portions of 500 mM urea solution to the cell. Every experiment was repeated three to six times for statistics. Non-specific changes in the output signal associated with fluctuations of temperature, medium pH, and electrical noise were avoided due to the usage of a differential measurement mode.
Effect of parameters of BEA and LTA zeolites (crystal size, Si/Al ratio, and surface functional groups) on biosensor operation
The observed more significant decrease in sensitivity to urea upon using LTA samples with respect to BEA samples can be due to a more hydrophilic nature of LTA samples (SiO2/Al2O3 ratio is approximately 1 for LTA and approximately 45 for BEA) and/or to noticeably bigger particle sizes (4 to 22 μm for LTA and 0.4 μm for BEA). The bigger particles have smaller surface area as in the case of LTA samples. Also, bigger zeolite particles could more efficiently prevent the substrate to reach the enzymatic sites. It seems that Si/Al ratio does not have a considerable effect on the biosensors in the current methodology of the biomembrane preparation. Possibly, the enzymes are not in close contact with the zeolites, and the zeolite particles act only like a diffusion barrier. Thus, the particle sizes might be more important for the biosensor sensitivity.
Effect of multilayer immobilization on function of clinoptilolite-based biosensors
At this stage, the main objective was to study the changes in characteristics of the biosensors upon addition of clinoptilolite to their bioselective elements.
In these experiments, an increase in the biosensor sensitivity was obtained using immobilization of the enzyme together with zeolite in a mixture. Further, we proposed two-layer immobilization using different combinations and ratios of zeolite and enzyme in the biomembrane. In the first case, 5% urease was deposited on the enzyme membrane and 10% BSA - on the reference membrane, and afterwards, immobilization was performed, and the sensor was washed to remove the GA excess; the second layers deposited on the same sensor were: 10% zeolite - on the enzyme membrane and 5% BSA - on the reference membrane.
As seen, when the enzyme is deposited on the sensor surface as the first layer and zeolite - as the second, the sensor sensitivity (curve 3) almost does not change compared to the urea sensor without zeolite (curve 1). Deposition of 5% zeolite as the first layer and 5% urease as the second (curve 4) increases the sensor responses by more than 1.5 times, and they are similar to the responses of the biosensors with single-layer membranes with a mixture of the enzyme and zeolite (curve 2). Deposition of 5% zeolite as the first layer and a mixture of 5% zeolite and 5% urease - as the second, results in even more significant increase in the biosensor responses (curve 5). However, a further increase of zeolite content in the membrane did not cause noticeable changes in the biosensor sensitivity (curve 6).
The results obtained can be explained by the fact that the presence of zeolite in the first layer improved diffusion properties of the membrane, which was a cause of higher sensitivity. Addition of zeolite in a larger concentration did not improve the sensor characteristics.
Effect of BEA thermal modification on the functioning of biosensors in direct determination of substrates and inhibitory analysis of toxic substances
Before performing the experiments on application of the developed biosensors for inhibitory determination of toxic substances, it was necessary to confirm that a decrease in the biosensor response to the substrate observed after inhibition in the tested solution was due to the inhibition of the bioselective element and not a result of the error of measurement. For this purpose, operational stability, an important working characteristic of biosensors, was investigated. The biosensor responses to 5 mM BuChCl and urea were measured within one working day with 30-min intervals, during which ISFETs with immobilized biomembranes were kept in the working buffer solution. All biosensors based on zeolite/enzyme demonstrated high signal reproducibility. Additionally, the experiments on storage stability were performed. The biosensors were stored in the buffer solution at room temperature, and all samples containing zeolite Beta were stable for more than 5 days.
In the work presented, a number of potentiometric biosensors based on pH-ISFET and different types of zeolite have been developed. Their working characteristics have been determined and compared.
The biosensors based on BEA showed higher activity and better sensitivity to substrate compared with the biosensors based on LTA. The best results were obtained for BEA-40 and BEA-50, which are more hydrophobic than BEA-30. The biosensors based on zeolite BEA-40 and BEA-50 demonstrated also a certain increase in the dynamic range of urea determination. The obtained results indicate that Si/Al ratio and particle diameter influence the biosensor characteristics. As the Si/Al ratio increases, hydrophobicity increases too, and the dynamic range of the determination becomes wider. The possible explanation of this phenomenon is the formation of hydrophobic interaction between the enzyme and BEA samples that have higher Si/Al ratio. The particle diameter has a slight influence on the biosensor parameters.
LTA has relatively small pore sizes; thus, the enzyme molecules can interact only with an external surface of the zeolite particles. Since the LTA-4 and LTA-9 particles were bigger compared to LTA-22, the latter had lesser amount of domains to interact with the enzyme.
The working characteristics of potentiometric biosensors with clinoptilolite-containing membranes were also investigated. The optimum concentration of zeolites in bioselective elements, at which the highest biosensor sensitivity was observed, was found to be 10%. A variety of designs of bioselective elements with clinoptilolite were examined; the two-layer biomembrane (the first layer - 5% zeolite, the second - a mixture of 5% zeolite and 5% enzyme) was stated to be the most promising.
The surfaces of ISFETs were modified by incorporation of the heat-treated zeolite Beta crystals. The employed heat treatment procedures affected the amount of Brønsted acid sites, whereas the amount of terminal silanols in zeolite Beta did not change. Additionally, these procedures did not impact on the size, morphology, Si/Al ratio, and external specific surface area of zeolite Beta particles. The zeolite addition improved the biosensor sensitivity, and the responses increased with an increase of the zeolite Brønsted acidity. The inhibition characteristics obtained for the zeolite-modified biosensors also correlated with the amount of Brønsted acid sites created in the samples upon heat treatment. Thus, the results presented here show that the interactions between the enzymes and the Brønsted acid sites of a zeolite support can affect the actual biosensor performances.
These results show that it is possible to regulate the ISFET characteristics for different enzyme-based biosensors by tailoring the electrode surfaces via various zeolites. This makes zeolites strong candidates for integration into biosensors as ISFET modifiers.
The authors gratefully acknowledge the financial support of this study by the Project European IRSES-NANODEV. National Academy of Sciences of Ukraine supported a part of this work in the frame of Scientific and Technical Government Program “Sensor systems for medico-ecological and industrial-technological requirement: metrological support and experimental operation”.
- Lee WY, Lee KS, Kim TH, Shin MC, Park JK. Microfabricated conductometric urea biosensor based on sol–gel immobilized urease. Electroanalysis. 2000;12:78–82.View ArticleGoogle Scholar
- Lee W, Kim S, Kim T, Lee KS, Shin M, Park J. Sol–gel-derived thick-film conductometric biosensor for urea determination in serum. Anal Chim Acta. 2000;404:195–203.View ArticleGoogle Scholar
- Jaffrezic-Renault N, Dzyadevych SV. Conductometric microbiosensors for environmental monitoring. Sensors. 2008;8:2569–88.View ArticleGoogle Scholar
- Adhikari B, Majumdar S. Polymersin sensor applications. Prog Polym Sci. 2004;29:699–766.View ArticleGoogle Scholar
- Bayramoğlu G, Yalçin E, Arıca MY. Immobilization of urease via adsorption onto l-histidine-Ni(II) complexed poly(HEMA-MAH) microspheres: preparation and characterization. Process Biochem. 2005;40:3505–13.View ArticleGoogle Scholar
- Chinnayelka S, McShane MJ. Microcapsule biosensors using competitive binding resonance energy transfer assays based on apoenzymes. Anal Chem. 2005;77:5501–11.View ArticleGoogle Scholar
- Wang J. Carbon-nanotube based electrochemical biosensors: a review. Electroanalysis. 2005;17:7–14.View ArticleGoogle Scholar
- Biloivan OA, Rogaleva NS, Korpan YI. Optimization of bioselective membrane of amperometric enzyme sensor on basis of glucose oxidase using NH2-modified multi-wall carbone nanotubes. Biopolym Cell. 2010;26:56–61.View ArticleGoogle Scholar
- Soy E, Arkhypova V, Soldatkin O, Shelyakina M, Dzyadevych S, Warzywoda J, et al. Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals. Mater Sci Eng C. 2012;32:1835–42.View ArticleGoogle Scholar
- Kucherenko IS, Soldatkin OO, Kasap B, Öztürk S, Akata B, Soldatkin AP, et al. Elaboration of urease adsorption on silicalite for biosensor creation. Electroanalysis. 2012;24:1380–5.View ArticleGoogle Scholar
- Soldatkin OO, Soy E, Errachid A, Jaffrezic-Renault N, Akata B, Soldatkin AP, et al. Influence of composition of zeolite/enzyme nanobiocomposites on analytical characteristics of urea biosensor based on ion-selective field-effect transistors. Sensor Lett. 2011;9:2320–6.View ArticleGoogle Scholar
- Shelyakina MK, Soldatkin OO, Arkhypova VM, Kasap BO, Akata B, Dzyadevych SV. Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors. Nanoscale Res Lett. 2014;9:124.View ArticleGoogle Scholar
- Carvalho RH, Lemos F, Cabral JMS, Ramôa RF. Influence of the presence of NaY zeolite on the activity of horseradish peroxidase in the oxidation of phenol. J Molecular Catal B. 2007;44:39.View ArticleGoogle Scholar
- Goriushkina TB, Akata Kurç B, Sacco Jr A, Dzyadevych SV. Application of zeolites for immobilization of glucose oxidase in amperometric biosensors. Sens Electron Microsystem Technol. 2010;1:36–43.Google Scholar
- Soldatkin OO, Ozansoy Kasap B, Akata Kurc B, Soldatkin AP, Dzyadevych SV, El'skaya AV. Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation. Biopolym Cell. 2014;30:291–8.View ArticleGoogle Scholar
- Kirdeciler SK, Soy E, Ozturk S, Kucherenko I, Soldatkin O, Dzyadevych S, et al. A novel urea conductometric biosensor based on zeolite immobilized urease. Talanta. 2011;85:1435–41.View ArticleGoogle Scholar
- Soldatkin OO, Kucherenko IS, Marchenko SV, Ozansoy Kasap B, Akata B, Soldatkin AP, et al. Application of enzyme/zeolite sensor for urea analysis in serum. Mater Sci Eng C. 2014;42:155–60.View ArticleGoogle Scholar
- Higgins JB, LaPierre RB, Schlenker JL, Rohrman AC, Wood JD, Kerr GT, et al. The framework topology of zeolite beta. Zeolites. 1988;8:446–52.View ArticleGoogle Scholar
- Creyghton EJ, Ganeshie SD, Downing RS, van Bekkum H. Stereoselective Meerwein-Ponndorf-Verley and Oppenauer reactions catalyzed by zeolite BEA. J Mol Catal A. 1997;115:457–72.View ArticleGoogle Scholar
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