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Table 1 Primary antibodies and antigen retrieval methods used in immunohistochemical evaluation

From: The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

Immunohistochemistry
Primary antibody	Clonality	Antigen unmasking	Dilution	Producer
Ki-67, clone MIB-1	Monoclonal	Microwave, citrate buffer, pH 6.0	1:50^a	Dako
Active caspase 3	Polyclonal	Water bath, citrate buffer, pH 6.0	1:800^b	Cell Signaling
Active caspase 9	Polyclonal	Microwave, EDTA buffer, pH 8.0	1:40^a	Novocastra

^a1-h incubation, RT.
^bOvernight incubation, 4°C.

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