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Table 1 Primary antibodies and antigen retrieval methods used in immunohistochemical evaluation

From: The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

Primary antibody Clonality Antigen unmasking Dilution Producer
Ki-67, clone MIB-1 Monoclonal Microwave, citrate buffer, pH 6.0 1:50a Dako
Active caspase 3 Polyclonal Water bath, citrate buffer, pH 6.0 1:800b Cell Signaling
Active caspase 9 Polyclonal Microwave, EDTA buffer, pH 8.0 1:40a Novocastra
  1. a1-h incubation, RT.
  2. bOvernight incubation, 4°C.