Fluorescence and bar graph of AgNP- and AgPyNP-treated RAW264.7 cells. (a) Fluorescence microscopic analysis of ROS generation in AgNP- and AgPyNP-treated RAW264.7 cells and scale bar represents 35 μm. (b) Bar graphs represent the mean ± SD intensity values of triplicate treatment groups to analyse the ROS generation in AgNP- and AgPyNP-treated RAW264.7 cells. Error bars represent the standard deviations of three independent experiments. Student’s t-test was used to determine the statistical significance: **P < 0.01; ***P < 0.001. Presented data were combined from at least three experiments. AgNPs, silver nanoparticles; AgPyNPs, nanosilver-pyridoxine complexes.