Fig. 4
a Agarose gel electrophoresis showing retardation of pGL3 plasmid DNA by Pluronic-PEI-DR5-TAT at different N/P ratios, 0:1 (DNA only), 1:1, 2:1, 4:1, 6:1, and 8:1. b Pluronic-PEI-DR5-TAT protects plasmid DNA DNase I-mediated degradation at various concentrations was estimated by agarose gel electrophoresis. Naked DNA was used as control. c Influence of dithiothreitol on Pluronic-PEI-DR5-TAT/DNA complexes at various N/P ratios (4:1, 6:1, 8:1). The last three lanes were treated with 10 mM DTT