The Potential Application of Raw Cadmium Sulfide Nanoparticles as CT Photographic Developer
- Qiang Wu†1,
- Lingxin Huang†1,
- Zhan Li2,
- Wenzhen An3,
- Dan Liu1,
- Jin Lin1,
- Longlong Tian1,
- Xinling Wang4,
- Bo Liu1,
- Wei Qi5Email author and
- Wangsuo Wu1Email author
© Wu et al. 2016
Received: 6 January 2016
Accepted: 13 April 2016
Published: 28 April 2016
With the development of science and technology, new applications about nanoparticles should be explored to achieve full-scale knowledge. Therefore, in this work, the toxicity and potential application of raw cadmium sulfide nanoparticles (CdS) in vivo were further studied through ICP-OES and CTs. Surprisingly, CdS exhibited an excellent photographic property, except for finding the accumulation of CdS in the lungs, liver, spleen, and kidney with a strong dependence on time; it is also found that there were a significant uptake in the pancreas for an obvious CT imaging. And the following investigations showed that the raw CdS could damage the tissues accumulating nanoparticles. Through this work, it can be seen that the raw CdS being modified might be an excellent photographic developer for detecting cancers or other diseases.
KeywordsBiodistribution Damage CdS Mice
Cadmium sulfide nanoparticles (CdS), especially their quantum dots (QDs) have been raised a great deal of attention as a special class of nanoparticles (NPs), due to their fluorescence and semiconductor properties . At the same time, for their excellent luminescence, continuous excitation spectrum, controllable and narrow emission bands, and ease of the functionalization for tissue targeting, they show a great promise for medical imaging and treatment of disease . However, before those nanoparticles are applied in medical field widely, the safety assessment of biology and environment needs to be investigated detailedly. Thus, a large amount of investigations on toxicity of CdS have been carried out for this purpose in vivo and in vitro. Buffet et al.  examined the toxicity effects of CdS-engineered nanoparticles compared to soluble Cd, on marine ragworms exposed for 14 days to these contaminants (10 μg Cd L−1) in seawater or via food, and they pointed out oxidative processes as the main consequences of exposure to Cd-based NPs in worms. Domingos et al.  found that CdS exerted higher toxicity compared to the same amounts of soluble Cd on bacteria and algae, suggesting a specific nanoeffect. King-Heiden et al.  also indicated that CdS also produced distinctly different toxicity that could not be explained by Cd release. Using Cd2+ ions, they found that zebrafish larvae showed clear signs of Cd toxicity. However, nanoparticles were even more potent and produced end points of toxicity distinct from that of Cd2+. But it was also reported that the cadmium from the degradation of CdS could be redistributed over time. Yang et al.  and Rzigalinski et al.  have reviewed early mouse studies of toxicity of Cd-based QDs, which all showed the absence of any significant toxicity at low dosage.
It is reported that the primary mechanism for CdS cytotoxicity was introduction of free radical formation [8, 9]. Active cadmium-based QDs core did participate in radical formation. At the cellular level, cadmium induced oxidative stress by depletion of endogenous antioxidants such as glutathione and was associated with mitochondrial damage, induction of apoptosis, and disruption of intracellular calcium signaling . Where HepG2 cells were treated with different concentrations of Cd, a rapid and transient ROS generation had triggered Cd-induced apoptosis. Luo et al.  suggested that cadmium-containing QDs caused an increase of intracellular ROS levels in mouse renal adenocarcinoma (RAG) cells and induced autophagy and subsequent apoptosis. Li et al.  elucidated the relationship between Cd2+ and oxidative stress with experiments of biosurfactant-stabilized CdS (bs-CdS). They observed that bs-CdS had the capacity to generate free radicals indirectly and induced oxidative stress and apoptosis by releasing Cd2+ in cells. Singh et al.  synthesized highly stable and surface-protected CdS induced apoptotic cell death by selectively generating excess ROS in human prostate cancer lymph node carcinoma of the prostate (LNCaP) cells.
As well known, clinically, the frequently used CT photographic developer was iodine-containing compounds for the high density and low toxicity of iodine. But most of the iodine-containing compounds existed various defects, such as difficult to synthesize, non-targeting, and rapid metabolism. And so, people made a lot of exploratory development by using carbon nanoparticles to seek new photographic materials [14–16], but there also a problem that some special ions or groups must be linked to the surface of nanoparticles to achieve image effect in vivo. With a band gap of 2.4 eV and high electron mobility, CdS has high photocatalytic reactions and photoenergy conversion efficiency. And so, CdS has been generally considered as a strong candidate for high efficiency visible-light-driven photocatalysts . As the same mechanism for CT photographic developer, CdS nanoparticles may be an excellent CT photographic developer. However, there are rare reports about the potential application of CdS nanoparticles in CT photographic developer. Therefore, in this experiment, the raw cadmium sulfide nanoparticles (CdS) are injected intravenously to the mice to determinate the property of photographic development by CT, and also to evaluate their acute toxicological and pathological effects in vivo. Through this work, it can provide basic data in vivo of CdS to help doctors alleviate the negative effects of Cd-containing nanoparticles and facilitate comprehensive utility of Cd-containing nanoparticles in treatment and diagnosis of disease.
The raw CdS were purchased from Shanghai Biological Technology Co., Ltd, with particle size about 1–30 nm. And the CdS were characterized by XRD (D5000, Siemens, Germany), Jeol2010 TEM (Jeol, USA), TGA, and Raman spectrum Luminescence spectrometer (LS55, Perkin Elmer, USA). At the same time, the UV-visible spectrum of CdS nanoparticles was recorded with a Perkin-Elmer Lambda 25 spectrophotometer. And the CdS were prepared 2.5 g/L suspension by PBS. All chemical reagents were analytically pure unless specified otherwise.
Biodistribution of CdS in Mice
Kunming mice (female to male = 1:1) initially weighing 15 to 18 g were provided by Laboratory Center for Medical Science, Lanzhou University, Gansu, China. All animals were housed in individual cages in a temperature (21 to 22 °C) and light (from 0800 to 2000 h) controlled environment and were fed food and tap water ad libitum. All animal protocols were in accordance with the European Communities Council Directive of November 24, 1986 (86/609/EEC), and approved by Institutional Animal Care and Use Committees of Gansu Province Medical Animal Center and Lanzhou University Animal Committees Guideline. All mice (about 40 mice) were injected intravenously about 400 μg/mouse CdS solution. And the exposure groups of mice (six mice) were sacrificed at 1, 6, 16, 24, and 48 h, respectively, and then the blood (1 mL), heart, liver, spleen, lungs, and kidney were harvested and weighed. Then the selected tissues were digested and diluted to a certain concentration . At last, the Cd content of the solution was measured through ICP-OES (ICP3000). Moreover, the mice after exposure CdS about 2 and 6 h were provided to CT with dual-energy spectral CT imaging mode (CT from Lanzhou University NO2 Hospital, Discovery CT 750HD, GE healthcare). The GSI scan parameters were as follows: Prep Group,30 s; scan mode, axial; gantry rotation speed, 0.5 s/circle; tube voltage, 140 and 80 kV; tube current, 630 mA s; detector coverage,20 mm;30 %ASIR; matrix size,512; slice thickness, 0.625 mm. Through those experiments, the biodistribution and photographic property of CdS in mice were determined, and the detailed information was exhibited as the “Result and Discussion” section.
The Exposure Dosage Effect on Biochemical Indexes
The mice (six mice/group) were exposed to 0, 100, 200, and 400 μg/mouse CdS solution, respectively. Then, the exposure mice were sacrificed at 24 h later, and the blood was collected to obtain serum. The collected blood was stayed in room temperature about 15 min, and then were centrifuged at 4000 rpm about 10 min, the supernatant (serum) were collected and kept in 4 °C refrigerator. At the next day, the blood urea nitrogen (BUN), creatinine (CREA), cystatin-C (Cys-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TB) contents in serum were measured by ELISA kit (purchasing from Elabscience Biotechnology Co., Ltd). Through the above experiment, the effect of the exposure dosage of CdS on biochemical indexes were investigated.
The exposure groups of mice were sacrificed at about 24 h, and tissues such as the heart, liver, spleen, lungs, kidney, and pancreas were collected and the fixed right lobe from animals in each group was embedded in paraffin, sectioned onto slides, and stained with hematoxylin and eosin (H&E). H&E-stained slides were qualitatively analyzed for indications of inflammation and injury by a certified veterinary pathologist who was blinded to the treatment groups.
Results and Discussion
Biodistribution of CdS in Mice
However, it was interesting that the CT imaging of CdS showed an obvious absorption in the lungs, liver, spleen, kidney, and bladder, especially in the pancreas (Fig. 6), as we know, the phenomenon about the presence of nanoparticles in the pancreas was found firstly in this work. Therefore, it should be discussed how CdS could enter into the pancreas. As early as 1901 year, Opie et al. reported the common-channel hypothesis as the potential triggering mechanism for gallstone-induced pancreatitis . They found the pancreatic duct and the common bile duct communicated, and called this communication as a common channel, which could have allowed for the bile to enter into the pancreas. Accordingly, they proposed that the reflux of the bile through the common channel into the pancreatic duct represents the triggering event for biliary pancreatitis. What is more, it was reported that the gallstone could stimulate Oddi’s sphincter during rolling into the duodenum leading to congestion, edema, and spasm, and then resulted in the function disorder of Oddi’s sphincter, so far as to reverse shrink, which could produce bile or duodenum content regurgitation . Therefore, authors thought that CdS could enter into and damage the liver after exposure to mice, and parts of CdS might enter into the bile duct with bile, and then further into the duodenum. In this process, the CdS stimulated Oddi’s sphincter and triggered function disorder, resulting the CdS enter into the pancreas with bile or duodenum content regurgitation into the pancreas. And so, there was a high uptake of CdS in the pancreas (Fig. 6).
The Exposure Dosage Effect on Biochemical Indexes
From this work, it could be seen that the CdS exhibited an excellent property of CT photographic developer with a certain toxicity in vivo. CdS were mainly retained in the lungs, but slight in the liver, spleen, and kidney, with a strong dependence on time. In addition, the accumulation of CdS in the pancreas was found firstly, and authors gave a detailed discussion about this point. Accordingly, the biochemical indexes and histology sections also indicated that the CdS had caused serious damages to the tissues. Through this work, it could help doctors alleviate the negative effects of Cd-containing nanoparticles and facilitate comprehensive utility of Cd-containing nanoparticles in medicine.
This work was supported by the National Science Foundation of China NO. J1210001 and 21327801.
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