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Fig. 2 | Nanoscale Research Letters

Fig. 2

From: Tissue Plasminogen Activator Binding to Superparamagnetic Iron Oxide Nanoparticle—Covalent Versus Adsorptive Approach

Fig. 2

Determination of the tPA binding efficiencies on SPIONs by measurement of the remaining tPA activity in the supernatant of the reactions. a, b Coomassie and silver staining after SDS-PAGE of the supernatants. c, d Remaining tPA activity was measured with the chromogenic S-2288 activity assay. c Kinetic of the reaction supernatant of covalent and non-covalent functionalized SPIONs determined by the hydrolyzation of S-2288 and the arising p-nitroaniline absorption. d tPA activity calculated by the absorption change of p-nitroaniline emerging within the first 2 h during the kinetics shown in (c)

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