Fig. 2

Dose dependence of nanoclay particle-induced liver injury. Serum levels of the liver enzymes alanine aminotransferase (ALT) (a) and aspartate aminotransferase (AST) (b) were determined using commercially available kits (see Biochemical analyses section) 24 h after intravenous administration of nanoclay particles at the indicated doses. Histologic analyses of tissues of nanoclay particle-treated mice. At 24 h after administration of vehicle (c) or nanoclay particles (d), tissues were collected, fixed with 4% paraformaldehyde, sectioned, and stained with hematoxylin and eosin. Data are presented as mean ± standard error of the mean (n = 4). Significant difference (*P < 0.05, **P < 0.01) compared with the vehicle-treated group