Fig. 5

Cellular uptake and targeting efficiency of coumarin-6 in liposomes. a CLSM images of MOVAS cells pre-treated for 24 h with PDGF-BB (20 μg/mL), followed by incubation with anti-ICAM-1-coumarin-6 liposomes, or coumarin-6 liposomes for 4 h. Left column DAPI channel showing blue fluorescence, middle column coumarin-6 channel showing green fluorescence from the liposomes, right column merged channel showing DAPI and coumarin-6. Bars = 50 μm. b Flow cytometry analyses of MOVAS cells pre-treated for 24 h with PDGF-BB (20 μg/mL) followed by treatment with anti-ICAM-1-coumarin-6 liposomes or coumarin-6 liposomes for 4 h. Emitted light was collected at FL6 using a 660/20-nm band pass filter. c Mean coumarin-6 fluorescence intensities obtained from MOVAS cells pre-treated for 24 h with PDGF-BB (20 μg/mL) and incubated with anti-ICAM-1-coumarin-6 liposomes or coumarin-6 liposomes for 4 h. Values are expressed as the means ± SD (n = 3); **P < 0.01