Fig. 6

Cell proliferation assessments using the CCK-8 assay and BrdU immunofluorescence (a). The CCK8 proliferation assay showed that anti-ICAM-1-T0901317 liposomes prevented the proliferation of vascular smooth muscle cells (VSMCs) induced by PDGF-BB more substantially than T0901317 liposomes and T0901317. b Confocal images showing that administration of anti-ICAM-1-T0901317 liposomes decreased the number of BrdU+ cells following treatment with PDGF-BB (20 μg/mL) for 24 h more substantially than T0901317- liposomes or T0901317. Bars = 100 μm. c Graphs of BrdU-positive ratios. All the experiments were repeated three times and represented as the means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001