Table 5 A comparison of the developed DNA biosensor performance with other previously reported electrochemical DNA biosensor for the determination of pathogenic V. cholere DNA
Material and electrode design | Linear range (M) | LOD (M) | Hybridi-sation time (min) | Reference |
|---|---|---|---|---|
DNA-PSA-AuNPs-carbon SPE (Direct immobilisation of capture probe on PSA gold-matrix) | 1.0 × 10−21–1.0 × 10−8 | 1.0 × 10−21 | 60 | Present work |
AuNPs-PSA-Avidin conjugate label on reporter probe | 1.0 × 10−18–1.0 × 10−15 | 1.0 × 10−15 | 30 | Liew et al. (2015) [24] |
AuNP-latex label on DNA reporter probe (Avidin-biotin binding) | 1.0 × 10−15–1.0 × 10−12 | 1.0 × 10−16 | - | Kuan at el. (2013) [30] |
AuNP-latex label on DNA reporter probe (Avidin-biotin binding) | 1.0 × 10−12–1.0 × 10−9 | 0.5 × 10−16 | 20 | Pinijsuwan et al. (2008) [29] |
AuNP-latex label on DNA target (streptavidin-latex/biotin-AuNP) | 1.0 × 10−12–1.0 × 10−9 | 1.0 × 10−12 | 20 | Kawde & Wang (2003) [35] |