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A Resumable Fluorescent Probe BHN-Fe3O4@SiO2 Hybrid Nanostructure for Fe3+ and its Application in Bioimaging

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Abstract

A multifunctional fluorescent probe BHN-Fe3O4@SiO2 nanostructure for Fe3+ was designed and developed. It has a good selective response to Fe3+ with fluorescence quenching and can be recycled using an external magnetic field. With adding EDTA (2.5 × 10−5 M) to the consequent product Fe3+-BHN-Fe3O4@SiO2, Fe3+ can be removed from the complex, and its fluorescence probing ability recovers, which means that this constituted on-off type fluorescence probe could be reversed and reused. At the same time, the probe has been successfully applied for quantitatively detecting Fe3+ in a linear mode with a low limit of detection 1.25 × 10−8 M. Furthermore, the BHN-Fe3O4@SiO2 nanostructure probe is successfully used to detect Fe3+ in living HeLa cells, which shows its great potential in bioimaging detection.

Background

The development of new methods to detect all kinds of small molecules and ions has become an important task for scientific researchers. As one of the indispensable important metal ions in metabolic processes, Fe3+ plays an essential and crucial role in a variety of biological processes such as brain function and pathology, gene transcription, immune function, and mammalian reproduction [1,2,3,4,5,6,7,8,9]. The medical investigations indicate that the metabolic or biological processes are normal for the proper functioning of all living cells only when the Fe3+ concentration is in a suitable range. When Fe3+ concentration in a living body deviates from its suitable range, some diseases or serious disorders can be induced in the metabolic or biological processes [10,11,12]. Even though a variety of detection methods has been developed to detect Fe3+ [13,14,15], fluorescent technique is the more effective and powerful method, because of their operational simplicity, high sensitivity and selectivity, and low detection limit [16,17,18,19,20].

In these molecule-based fluorescent probes, some problems relative to the safety, the recyclability, and the reusability have not been solved. For example, as pointed out in reference [21], the employed small molecules are toxic. These deficiencies exhibited in the molecule-based fluorescent probes completely limit the probes entering into a practical application. To conquer the challenge of safety in the above fluorescent probes for Fe3+, another technical approach is proposed by using inorganic supports incorporated with small molecular fluorescent probes. In such new approach, it is known that the inorganic materials such as magnetic nanoparticles, metal nanoparticles, nanotubes, and mesoporous silica can be used in the design of the fluorescent probes [22,23,24]. Among all these inorganic materials, magnetic silica core-shell Fe3O4@SiO2 nanoparticles have advantages of their low toxicity, high biocompatibility, simply separation via external magnetic field, and large surface area that can be grafted by fluorescent probes over other materials in the molecule or ion recognition and separation areas [25,26,27]. Hence, this new approach provides us a possible way to realize the application for detecting Fe3+, especially in the safety with low toxicity and high biocompatibility.

In this work, a kind of multifunctional magnetic BHN-Fe3O4@SiO2 nanostructure fluorescent sensor for Fe3+ was designed and synthesized. It has a good sensitive and selective response to Fe3+ with remarkably fluorescence quenching in CH3CN/H2O (1:1, v/v) at room temperature. By applying an external magnetic field, the probe can be separated from the solution. When adding EDTA to the system, Fe3+ can be removed from the complex with fluorescence intensity recovery. Furthermore, the confocal fluorescence imaging using HeLa cells showed that the probe could be applied to detect Fe3+ in living cells. Hence, the obtained BHN-Fe3O4@SiO2 exhibits excellent selectivity, water solubility, reversibility, and recyclability, which benefits to the detection of Fe3+.

Methods/Experimental

Synthesis of Fe3O4@SiO2 Nanoparticles

Fe3O4 magnetite nanoparticles were synthesized according to reference [28]. They were further coated with a thin silica layer by means of a modified Stöber method [29] to obtain stable Fe3O4@SiO2. Tetraethyl orthosilicate (TEOS) was hydrolyzed with magnetite nanoparticles as seeds in ethanol/water mixture. The resulting Fe3O4@SiO2 nanoparticles with an average diameter of 50–60 nm were used as the carriers of fluorescent sensor nanoparticles.

Synthesis of BHN-Fe3O4@SiO2 Nanostructure

N-butyl-4-bis(2-hydroxyethyl) amino-1,8-naphthalimide (BHN) is synthesized according to the method reported before [30, 31]. The first intermediate was synthesized by the reaction between 4-bromo-1,8-naphthalic anhydride and n-butylamine. Then, the intermediate reacted with diethanolamine to afford BHN. ESI-MS: m/z 357.3 (M + H+). 1H NMR (CDCl3, 400 MHz): δ (ppm): 0.95 (t, 3H, J = 8.0 Hz); 1.41(m, 2H); 1.66 (m, 2H); 2.69 (m, 2H); 3.60 (t, 4H, J = 5.0 Hz); 3.86(t, 4H, J = 5.0 Hz); 4.08 (t, 2H, J = 8.0 Hz); 7.33 (d, 1H, J = 8.0 Hz); 7.58 (t, 1H, J = 8.0 Hz); 8.38(d, 1 H, J = 8.0 Hz); 8.41 (dd, 1H, J = 8.0 Hz); 8.84 (dd, 1H, J = 8.0 Hz).

BHN (356 mg, 1 mmol) and 3-isocyanatopropyl-triethoxysilane (IPTES, 494 mg, 2 mmol) were mixed in anhydrous THF (15 mL) at room temperature. Then the solution was refluxed for 48 h under N2. After that, the solvent was evaporated, and the crude product was further purified by flash column chromatography (silica gel, petroleum ether/CH2Cl2/methanol 50/50/1) to afford 255 mg (30%) of BHN-IPTES as a yellow powder. ESI-MS: m/z 851.5(M + H+). 1H NMR: (400 MHz, CDCl3): δ (ppm) 0.60 (t, 4H, J = 8.0 Hz); 0.98 (t, 3H, J = 8.0 Hz); 1.21 (m, 18H); 1.45 (m, 2H); 1.58 (m, 4H); 1.70 (m, 2H); 3.13 (m, 4H); 3.73 (t, 2H, J = 5.0 Hz); 3.82 (m, 12H); 4.16 (m, 4H); 4.24 (m, 4H); 4.94 (m, 2H); 7.38 (d, 1H, J = 8.0 Hz); 7.70 (t, 1H, J = 8.0 Hz); 8.45 (d, 1H, J = 8.0 Hz); 8.50 (dd, 1H, J = 8.0 Hz); 8.58 (dd, 1H, J = 8.0 Hz).

One hundred milligrams of dried Fe3O4@SiO2 nanoparticles and 300 mg (0.35 mmol) of BHN-IPTES were suspended in anhydrous toluene (15 mL). The solution was refluxed for 12 h at 110 °C under N2 to obtain BHN-Fe3O4@SiO2. The nanoparticles were collected by centrifugation (10,000 rpm) and repeatedly washed with anhydrous ethanol thoroughly. By monitoring the fluorescence of the upper liquid, unreacted organic molecules could be removed completely. Then, the BHN-Fe3O4@SiO2 nanostructure was finally dried under vacuum overnight.

Results and Discussion

Design of BHN-Fe3O4@SiO2

Fe3O4@SiO2 nanoparticle is a promising candidate to construct safe, recyclable, and reusable Fe3+ fluorescent sensor due to its low toxicity, high biocompatibility, and convenient recyclability via external magnetic field. Compared with other fluorophores, 1,8-naphthalimide has a large Stokes’ shift, long emission wavelength, and convenience to modify with different side-chain and high quantum yield. So, with the introduction of proper side-chain, it can be grafted on the Fe3O4@SiO2 nanoparticle to obtain a safe, recyclable, and reusable Fe3+ fluorescent sensor with remarkable fluorescence response.

As is well known, Fe3+ can be easily coordinated with O and N atom, so we modified 1,8-naphthalimide with diethanolamine to make the 1,8-naphthalimide possess the ability to detect Fe3+ as shown in Fig. 1a. In the diethanolamine, hydroxyethyl and ester-amide moieties were served as a receptor unit. Finally, the modified 1,8-naphthalimide was grafted on the Fe3O4@SiO2 via hydrolysis-condensation reaction between Si (OEt)3 and hydroxyl in the surface of Fe3O4@SiO2 as shown in Fig. 1b.

Fig. 1
figure1

a Synthesis of BHN. b Synthesis of BHN-Fe3O4@SiO2

Structure of BHN-Fe3O4@SiO2

From the TEM image as shown in Fig. 2a, the typical core/shell structure of BHN-Fe3O4@SiO2 is clearly displayed. Although the bare magnetic core is easy to aggregate in liquid, the silica shell on the surface of magnetic nanoparticles would prevent aggregation and improve the dispersibility. The iron oxide nanoparticles have been entrapped in the silica shell successfully and dispersed well. It can also be seen that the overall diameters of core/shell structures are in a narrow distribution of 50 to 60 nm with iron oxide core of 10 nm, which is lower than its superparamagnetic critical size and suitable for using as fluorescent probe’s carrier nanoparticle.

Fig. 2
figure2

a TEM image of BHN-Fe3O4@SiO2 (the scale bar is 50 nm.). b XRD patterns of Fe3O4/Citrate, Fe3O4@SiO2, and BHN-Fe3O4@SiO2. c FT-IR spectra of Fe3O4@SiO2 and BHN-Fe3O4@SiO2. d TG and DTA curves of Fe3O4@SiO2 and BHN-Fe3O4@SiO2

Figure 2b shows the XRD powder diffraction patterns of Fe3O4, Fe3O4@SiO2, and BHN-Fe3O4@SiO2. The six characteristic diffraction peaks of bare Fe3O4 can be indexed to 220, 311, 400, 422, 511, and 440 reflections of the magnetite. However, the XRD peaks attributed to Fe3O4 have low intensities in Fe3O4@SiO2 and BHN-Fe3O4@SiO2, which implies that the Fe3O4 nanoparticles are coated with amorphous silica shell. The silica shell may decrease the relative content of Fe3O4 cores and then affect the peak intensities. Also, the broad XRD package is found at a low diffraction angle of 20° to 30° in Fe3O4@SiO2 and BHN-Fe3O4@SiO2, which corresponds to the amorphous-state SiO2 shells surrounding the Fe3O4 nanoparticles.

To study the modification condition of BHN-IPTES on the surface of the Fe3O4@SiO2 nanoparticles, its Fourier transform infrared (FT-IR) spectrum is measured. As shown in Fig. 2c, both two curves exhibited the typical vibration band of −OH stretching on silanol at 3400 to 3500 cm−1 and 1000 to 1200 cm−1 [32]. It indicates that not all the silanol on Fe3O4@SiO2 nanoparticles have been covalently modified. The band at 1630 cm−1 represents the bending mode of −OH vibrations [33]. The bands centered at 1109 (νas) and 800 cm−1 can be attributed to the siloxane (-Si-O-Si-) [34]. The above peaks indicate the existence of silica shell. The additional peaks at 2965 and 2934 cm−1 were found in BHN-Fe3O4@SiO2, corresponding to the −CH vibration of aliphatic and aromatic groups [32, 35]. The band at 1697, 1590, and 1516 cm−1 of BHN-Fe3O4@SiO2 comes from the bending vibrations of −CH3 from the BHN part [36]. These results demonstrate the presence of the organic molecule in the magnetic material BHN-Fe3O4@SiO2.

The superparamagnetic property of the magnetic nanoparticles plays a vital role for its biological application. Additional file 1: Figure S1 shows the magnetization curve of BHN-Fe3O4@SiO2 which was measured by a vibrating sample magnetometer in the range from − 15,000 to 15,000 Oe at 300 K. The result was consistent with the conclusion that the diameter of magnetic Fe3O4 nanoparticles less than 30 nm is usually superparamagnetic at room temperature [37]. The saturation magnetization value for synthesized BHN-Fe3O4@SiO2 is about 4.02 emu/g. More importantly, from the hysteresis loop of BHN-Fe3O4@SiO2 nanostructure, it can be found that it exhibited superparamagnetic properties, and no coercive force was observed in the hysteresis loop. This phenomenon was due to the fact that the magnetite core has a small diameter around 10 nm. At the same time, the silica shell prevents the aggregation of magnetite core. So, the BHN-Fe3O4@SiO2 nanostructure can further show good dispersibility.

Fluorescence Response of BHN-Fe3O4@SiO2

To verify the fluorescence response of BHN-Fe3O4@SiO2 for various metal ions, the fluorescence measurements were carried out in CH3CN/H2O 1:1 (v/v) solution at pH 7.36 in HEPES buffer. The concentration of BHN-Fe3O4@SiO2 is 0.2 g/L (corresponding to the free organic molecule was about 3.34 × 10−5 M, according to the TGA, see Fig. 2d), and the various metal ions Ag+, Al3+, Ca2+, Cd2+, Co2+, Cr3+, Cu2+, Hg2+, K+, Li+, Mg2+, Mn2+, Na+, Pb2+, Zn2+, and Fe3+ (all as their perchlorates salts) were 5.0 × 10−5 M. As shown in Fig. 3a, a significant fluorescence quenching was observed when adding Fe3+, but no significant decrease of fluorescent intensity in the same conditions was detected if adding other metal ions except Cu2+. Cu2+ would cause slight fluorescence quenching and response in 20 min. However, at the same detecting conditions, Fe3+ causes a response in 2 min and quench obviously in 5 min (Fig. 3c). The absorption spectra of BHN-Fe3O4@SiO2 (0.2 g/L) in the presence of various concentrations of Fe3+ (0 to 200 μM) were investigated, as shown in Fig. 3d. When Fe3+ was added gradually, the absorbance of BHN-Fe3O4@SiO2 at 250 and 350 nm gradually increases, which indicated that BHN-Fe3O4@SiO2 nanostructure coordinated with Fe3+ gradually.

Fig. 3
figure3

a Fluorescence responses of BHN-Fe3O4@SiO2 with various cations. Excitation wavelength was 415 nm. Spectra were recorded every 2 min after adding metal ions. b Competition of Fe3+-BHN-Fe3O4@SiO2 towards cations. Fluorescent emission change of BHN-Fe3O4@SiO2 (0.2 g/L) upon addition of metal ions (each metal ion is 5 × 10−5 M) in CH3CN/H2O 1:1 (HEPES buffer pH 7.36) at room temperature. c Time responses of BHN-Fe3O4@SiO2 with Fe3+ and Cu2+. d UV-Vis titrations of BHN-Fe3O4@SiO2 (0.2 g/L) with Fe3+. e Fluorescence titration of BHN-Fe3O4@SiO2 (0.2 g/L) with Fe3+. Inset: the fluorescence intensities at 518 nm at various concentrations of Fe3+. f Job’s plot of BHN-Fe3O4@SiO2 with Fe3+

Then, a fluorescence titration with Fe(ClO4)3 in CH3CN/H2O 1:1 (v/v) was applied to understand the combination of BHN-Fe3O4@SiO2 towards Fe3+ ions. As illustrated in Fig. 3e, the fluorescence emission of BHN-Fe3O4@SiO2 (0.2 g/L) decreases gradually when various concentrations (0 to 100 μM) of Fe3+ were added in CH3CN/H2O 1:1 (v/v) HEPES buffer, which indicates that BHN-Fe3O4@SiO2 nanostructure coordinated with Fe3+ to form the complex quantitatively. Fluorescence titration experiment suggests that the association constant logβ for Fe3+ binding to BHN-Fe3O4@SiO2 is calculated to be 8.23. A linear increasing of fluorescence from the BHN-Fe3O4@SiO2 nanostructure was observed upon the addition of Fe3+ between 0 and 20 μM, and the limit of detection of BHN-Fe3O4@SiO2 to Fe3+ was found by 1.25 × 10−8 M under the fluorimetric assay. The fluorescence titration and Job plot results suggested a 1:1 binding ration for Fe3+ with BHN-Fe3O4@SiO2 (Fig. 3f). The results of cation competitive experiments are depicted in Fig. 3b, and it could be found that the selectivity and sensitivity of BHN-Fe3O4@SiO2 to Fe3+ are not influenced by other metal ions.

Here, the remarkable decrease of fluorescence intensity can be explained as follow: The fluorescence intensity of BHN-Fe3O4@SiO2, which is excited at a 415 nm lamp, exhibits the high fluorescence at 518 nm due to the 1,8-naphthalimide which has a big conjugated system. In addition, electron donating group in the structure influences the fluorescent of system at the same time. When stably chelated with Fe3+ by the O atom and N atom on the four-position of 1,8-naphthalimide, the electron or energy transfer between the metal cation and the fluorophore produce an electronic absorption effect, so as to make the fluorescence quenching [38] (Fig. 4a).

Fig. 4
figure4

a Schematic show of BHN-Fe3O4@SiO2 with Fe3+. b Reversibility of BHN-Fe3O4@SiO2 towards Fe3+. Inset: the photograph of BHN-Fe3O4@SiO2 with Fe3+ by treatment of EDTA (2.5 × 10−5 M) under 415-nm UV light. c Plot of the fluorescence of BHN-Fe3O4@SiO2 (0.2 g/L) with alternate adding of 2.5 × 10−5 M Fe3+ (“off”) and EDTA (“on”). d BHN-Fe3O4@SiO2 (0.2 g/L) was dispersed to an external magnet in CH3CN/H2O 1:1 (HEPES buffer pH 7.36)

The fluorescence quenching by adding Fe3+ to the solution of BHN-Fe3O4@SiO2 was fully reversible. When adding EDTA (2.5 × 10−5 M) to the Fe3+-BHN-Fe3O4@SiO2 system, the fluorescence intensity was almost restored to the original level of BHN-Fe3O4@SiO2 (Fig. 4b). Further, reusability was evaluated by repeatedly adding Fe3+-EDTA cycles into the system, with the change of BHN-Fe3O4@SiO2 fluorescence intensity being recorded after each step, and the corresponding data are shown in Fig. 4c. It is clear that the BHN-Fe3O4@SiO2 exhibits excellent reusability because only the rare loss in BHN-Fe3O4@SiO2 sensitivity towards Fe3+ was observed after five repeated Fe3+-EDTA cycles. As a result of its magnetic property, BHN-Fe3O4@SiO2 had a reversal magnetic responsibility. As shown in Fig. 4d, it could be easily separated from the dispersion (0.2 g/L) after 10 min by placing a magnet closed to the dispersion, then redispersed by mild agitation when the magnet was removed. This magnetic separation capability and the recognition property of BHN-Fe3O4@SiO2 nanostructure provide a simple and efficient route to separate Fe3+ rather than through filtration approach. More important is that the reversal magnetic responsibility of BHN-Fe3O4@SiO2 nanostructure would be a key factor when evaluating their recyclability [39]. Combined with its magnetic property, it is demonstrated that BHN-Fe3O4@SiO2 was considerably applicable in the biological system as an efficient inorganic-organic hybrid sensor for Fe3+.

For the biological application, it is critically important that the sensor should be suitable for measuring specific metal ion in the physiological pH range. As shown in Fig. 5a, the fluorescence intensities of BHN-Fe3O4@SiO2 with/without Fe3+ at various pH values were investigated. The fluorescence intensity of BHN-Fe3O4@SiO2 slightly decreases when adding Fe3+ under acidic conditions, since protonation of N atom on the four-position of 1,8-naphthalimide leads to a weak coordination ability of Fe3+. Then, a dramatic fluorescence change for Fe3+-BHN-Fe3O4@SiO2 system was found when pH was at neutral pH and under weakly alkaline conditions. Here, BHN-Fe3O4@SiO2 exhibits excellent Fe3+ sensing abilities when the pH is in the range of 5.84 to 10.52, which indicates that BHN-Fe3O4@SiO2 is an expecting probe to be applied in those complicated environments or biological systems.

Fig. 5
figure5

a Fluorescence intensities of BHN-Fe3O4@SiO2 and Fe3+-BHN-Fe3O4@SiO2 at various pH values at room temperature. CH3CN/H2O 1:1, λ ex = 415 nm. b Bright field image and fluorescence image of the HeLa cells with BHN-Fe3O4@SiO2. (c) Bright field image and fluorescence image of the HeLa cells with BHN-Fe3O4@SiO2 and Fe3+

To further demonstrate the ability of BHN-Fe3O4@SiO2 to detect Fe3+ in living systems, we carried on an experiment in live HeLa cells. First of all, we investigated the cell viability of BHN-Fe3O4@SiO2 and Fe3+-BHN-Fe3O4@SiO2 using the MTT assay. HeLa cells were incubated with BHN-Fe3O4@SiO2 in RPMI-1640 for 0.5 h at 37 °C, and then Fe(ClO4)3 was added for incubation for 0.5 h. Then, the confocal fluorescence images of the HeLa cells were observed, and it shows excellent staining capacity when the concentration of the sensor and Fe(ClO4)3 is up to 0.2 g/L and 5 × 10−5 M. Then, we conducted fluorescence microscopy experiment to investigate its higher gradation of application in complex biological systems. As shown in Fig. 5b, HeLa cells were grown on 12 orifice plate at 37 °C and in 5% CO2 atmosphere for 24 h, then treated with BHN-Fe3O4@SiO2 (0.2 g/L) and incubated for 0.5 h, and the cells showed strong green fluorescence. Then, the cells were treated with 5 × 10−5 M Fe(ClO4)3. After 0.5 h, we did observe the fluorescent remarkably decreased (Fig. 5c). Thus, we can draw a conclusion that BHN-Fe3O4@SiO2 can be used to image Fe3+ in living cells.

Conclusion

In summary, a novel multifunctional fluorescent probe BHN-Fe3O4@SiO2 nanostructure for Fe3+ was successfully designed and synthesized. The probe BHN-Fe3O4@SiO2 can selectively respond to Fe3+ with fluorescence quenching and efficient separation of Fe3+ with external magnetic field. The constituted on-off type fluorescence monitoring system indicates that the probe could be reversed back and reused. At the same time, the probe has been successfully applied to quantitatively detect Fe3+ with low detection limits. Furthermore, the BHN-Fe3O4@SiO2 nanostructure probe is successfully used to detect Fe3+ in living HeLa cells, which shows its great potential in bioimaging detection.

Abbreviations

BHN:

N-butyl-4-bis(2-hydroxyethyl) amino-1,8-naphthalimide

EDTA:

Ethylenediaminetetraacetic acid

FT-IR:

Fourier transform infrared

IPTES:

3-Isocyanatopropyl-triethoxysilane

TEM:

Transmission electron microscopy

TEOS:

Tetraethyl orthosilicate

TGA:

Thermal gravimetric analysis

THF:

Tetrahydrofuran

XRD:

X-ray powder diffraction

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Acknowledgements

This study was supported by the NSFC (NO. 20931003, 20771048) and the Specialized Research Funds for the Doctoral Program of Higher Education (20110211130002).

Availability of data and materials

All reagents are purchased commercially, and tetrahydrofuran (THF) and toluene were used after dehydration. Other chemicals were used as received without further purification. Thermal gravimetric analysis (TGA) was performed on a PE Diamond TG/DTA/SPAECTRUN ONE thermal analyzer up to 800 °C at a heating rate of 10 °C min−1 in an N2 atmosphere. Transmission electron microscopy (TEM) (Tecnai G2 F30, 300 kV, FEI Company, OR, USA) was used to characterize the materials. X-ray diffraction (XRD) pattern of the synthesized products was recorded with a Rigaku D/MAX 2400 X-ray diffractometer (Tokyo, Japan) using Cu radiation (λ = 0.154056 Å). The scan range (2θ) was from 10° to 80°. Solid-state infrared (IR) using diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy was performed in the 400 to 4000 cm−1 region using a Bruker Vertex 70 V (Bremen, Germany) and IR-grade KBr (Sigma-Aldrich Corporation, St. Louis, MO, USA) as the internal standard. 1H NMR was measured on a Bruker DRX 400 spectrometer in a CDCl3 solution with TMS as the internal standard. Chemical shift multiplicities are reported as s = singlet, t = triplet, q = quartet, and m = multiplet. Mass spectra were recorded on a Bruker Daltonics esquire6000 mass spectrometer. UV absorption spectra were recorded on Varian Cary 100 spectrophotometer (Palo Alto, CA, USA) using quartz cells of 1.0-cm path length. Fluorescence measurements were made on a Hitachi F-7000 spectrophotometer (Tokyo, Japan) and a Shimadzu RF-540 spectrofluorophotometer (Chorley, UK) equipped with quartz cuvettes of 1.0-cm path length with a xenon lamp as the excitation source. An excitation and emission slit of 5.0 nm was used for the measurements in the solution state. All pH measurements were made with a pH-10C digital pH meter. All spectrophotometric titrations were performed with a suspension of the sample dispersed in CH3CN/H2O (1:1, HEPES buffer pH 7.36).

Author information

XZ supervised and participated in all the studies and wrote this paper. YW conceived the study and participated in its design. QP participated in the cell experiment. WL participated in the revision of the manuscript. All authors read and approved the final manuscript.

Correspondence to Weisheng Liu.

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Additional file

Additional file 1: Figure S1. a

Magnetization curve of BHN-Fe3O4@SiO2. b UV-Vis spectra of BHN-IPTES, Fe3O4@SiO2, and BHN-Fe3O4@SiO2. (PDF 57 kb)

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Zhou, X., Wang, Y., Peng, Q. et al. A Resumable Fluorescent Probe BHN-Fe3O4@SiO2 Hybrid Nanostructure for Fe3+ and its Application in Bioimaging. Nanoscale Res Lett 12, 629 (2017) doi:10.1186/s11671-017-2392-2

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Keywords

  • Fluorescent probe
  • Resumable
  • Hybrid nanostructure
  • Fe3+
  • Bioimaging