Fig. 2
NaOH pretreatment is the optimal method for detecting nAg100 in mouse liver. Five solubilizing reagents were screened as pretreatment solvents to lyse the tissues (NaOH, TMAH, HNO3, HCl, and proteinase K). The liver homogenate was mixed with nAg100 solution to obtain a final Ag concentration of 100 ng/mL and treated with each solubilizing reagent at 37 °C. After 3 h, a residue rates in each group as an indicator of tissue solubility, b recovery rates (black and white bars represent the rate of silvers detected as particles and as ions, respectively), c average particle diameters shown in a bar chart, and d particle size distribution shown in a beeswarm chart were analyzed by sp-ICP-MS analysis. The results are expressed as mean ± SD (n = 3)