Fig. 1
a According to the z-axis scan of a gold thin film used to measure the signal of second harmonic generation at different positions, the z-axis resolution of the laser system (full width at half maximum) is approximately 0.90159 μm (fitting using Gaussian function). b TPL intensity dependence on the excitation power (logarithm) of the materials and fluorophores; TPE exposure from 704.0 to 2816.0 nJ pixel−1 for Rhodamine B and fluorescein, from 1408.0 to 2816.0 nJ pixel−1 for water-soluble C60(OH)21 fullerenol, and from 1760.0 to 2816.0 nJ pixel−1 for water-soluble C60(OH)46 fullerenol. Excitation wavelength, 760 nm. Delivered dose: OD600 0.05 of E. coli and 3 μg mL−1 materials. Data are presented as means ± SD (n = 6)