Fig. 1
From: Kinetics and Specificity of HEK293T Extracellular Vesicle Uptake using Imaging Flow Cytometry
Characterization of HEK293T EVs tagged with CD63-eGFP. EVs were isolated from HEK293T (control) and HEK293T expressing CD63-eGFP cell culture media. a Representative EV size distribution recorded via nanotracking software. b Quantification of mean diameter distribution of transfected vs. non-transfected HEK293T EVs. c IFC images of negative control beads, HEK293T control EVs, and CD63-eGFP–tagged EVs. BF signifies bright field, GFP signifies green fluorescent protein (488 nm excitation laser), and SSC signifies side scatter. Positive eGFP in GFP channel signifies fluorescent HEK293T EVs. d Flow cytometry-based MACSPlex surface marker expression of non-transfected HEK293T EVs and HEK293T CD63-eGFP EVs. Both EV sources are positive for CD29, CD9, CD63, and CD81, as measured in relative fluorescence (denoted by X for positive). Bars represent mean ± SEM; N = 3; unpaired T test. n.s. signifies p > 0.05