Fig. 3
From: Kinetics and Specificity of HEK293T Extracellular Vesicle Uptake using Imaging Flow Cytometry
HEK293T EV uptake has a dose effect with a minimum threshold of 6000 EVs. HEK293T cells were co-cultured with HEK293T EVS at increasing doses from 0 to 20,000/cell. a Representative IFC images of recipient cells with respective EV doses. GFP localization signifies HEK293T EV uptake. b–d Quantification of dose assays compared with controls and each group via spot count, mean fluorescence intensity, and maximum pixel ratios. Bars represent mean ± SEM; N = 3; one-way ANOVA followed with Tukey’s post hoc test. *p < 0.05; ***p < 0.01