Fig. 4
F-actin assembly regulated the expression of osteogenic genes in BMSCs. a, b Cell proliferation after Cyto D and Jasp treatment was determined using the CCK-8 assay or automated cell counter at days 1, 2, and 3. c, d ALP staining and ALP activity analysis was performed to evaluate the expression of ALP in MSCs after drug treatment for 3 days. Staining area was analyzed using ImageJ. c, e–h Western blotting and qRT-PCR were used to compare the changes of osteogenesis-related markers in the NT+ Cyto D group and the NT+ Jasp group with those in the control group (without drug treatment). NT the nanotubes group. Data represent the mean ± SD of three samples. *P < 0.05, **P < 0.01, and ***P < 0.001