Fig. 4

M1 macrophage exosomal miR-326 inhibits migration and invasion of HCC cells. a Transwell assay detected the effect of transfection of miR-326 mimic on the invasion of HepG2 cells. b Transwell assay detected the effect of transfection of miR-326 mimic the migration of HepG2 cells. c The number of invasion and migration of HepG2 cells. d Transwell assay detected the effect of transfection of miR-326 inhibitor on the invasion of SMMC-7721 cells. e Transwell assay detected the effect of transfection of miR-326 inhibitor on the migration of SMMC-7721 cells. f The number of invasion and migration of SMMC-7721 cells. g Transwell assay detected the effect of co-culture with miR-326 mimic-transfected M1 macrophage-derived exosomes on the invasion of HepG2 cells. h Transwell assay detected the effect of co-culture with miR-326 mimic-transfected M1 macrophage-derived exosomes on the migration of HepG2 cells. i The number of invasion and migration of HepG2 cells co-cultured with M1 macrophage-derived exosomes. j Transwell assay detected the effect of co-culture with miR-326 inhibitor-transfected M1 macrophage-derived exosomes on the invasion of SMMC-7721 cells. k Transwell assay detected the effect of co-culture with miR-326 inhibitor-transfected M1 macrophage-derived exosomes on the migration of SMMC-7721 cells. l The number of invasion and migration of SMMC-7721 cells co-cultured with M1 macrophage-derived exosomes. In panel c, *P < 0.05 versus the NC-mimic group; In panel f, *P < 0.05 versus the NC-inhibitor group; In panel i, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-mimic group; In panel l, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-inhibitor group. Measurement data were depicted as mean ± standard deviation (N = 3), comparisons among multiple groups were conducted by one-way analysis of variance