Fig. 6

SOX2 can bind to CCAT1 which affects miR-185-3p expression, and FOXP3 is a target gene of miR-185-3p. a Prediction of binding sites in SOX2 and CCAT1 promoter regions by bioinformatics sites. b ChIP-qPCR experiment verified the binding relationship between SOX2 and CCAT1. c The binding site of SOX2 and CCAT1 verified by dual luciferase reporter gene assay. d Prediction of binding sites in CCAT1 and miR-185-3p by bioinformatics sites. e Verification of the binding of CCAT1 and miR-185-3p by dual luciferase reporter gene assay. f The binding relationship between CCAT1 and miR-185-3p in cells verified by RNA pull-down assay. g Prediction of the targeting relationship between miR-185-3p and FOXP3 by bioinformatics website. h Identification of the targeting relationship between miR-185-3p and FOXP3 by dual luciferase reporter gene assay. Measurement data were depicted as mean ± standard deviation, and comparisons between two groups were assessed by t test