Fig. 2
From: Analysis of Actin and Focal Adhesion Organisation in U2OS Cells on Polymer Nanostructures
A Images of U2OS cells on different surfaces were acquired at three indicated focal planes. Images were obtained close to the pillar base, approximately at half-pillar height, and close to the pillar apex. The imaging planes were located 0.0 μm, 0.4 μm, 0.8 μm from the flat glass surface. B–D) Merged fluorescence images showing U2OS expressing fluorescent LifeActGFP (green) and TagRFP-vinculin (red) on different surface types, yellow colour is indicative of overlapping LifeActGFP and TagRFP-vinculin. Imaged surfaces were B non-structured glass surface, C 1000 nm pitched pillar array, D 2000 nm pitched pillar array. Presented images are representative for cells on each surface type. The vertical distance between focal planes for each surface type is approximately 400 nm. Scalebars 25 μm